TITLE:
Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer
AUTHORS:
O. Suvorova, A. Perchik, M. Slyadnev, O. Suvorova, A. Perchik, M. Slyadnev, D. Navolotskii, N. Mushnikov
KEYWORDS:
Polymerase Chain Reaction; Sexually Transmitted Infections; Microchip
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
January
16,
2013
ABSTRACT: Development of non expensive and time-saving
techniques based on the polymerase chain reaction (PCR) is of great importance
for modern diagnostics. We considered a new approach for PCR determination of a
variety of sexually transmitted infections using microchip analyzer “AriaDNA”,
which had been tested using clinical samples in several medical institutions of
St. Petersburg (Russia). The use of microchips containing lyophilized PCR
reagents allows reducing significantly time of analysis and the number of
manipulations thus preventing possible sample contamination.