Wachsman, M.B., Castilla, V., Talarico, L.B., Ramirez, J.A., Galagovsky, L.R. and Coto, C.E. (2004) Antiher-patic mode of action of (22S, 23S)-3beta-Bromo-5alpha, 22, 23-trihydroxystigmastan-6-one in vitro. International Journal of Antimicrobial Agents, 23(5), 524-526.

has been cited by the following article:

• TITLE: Inhibition of H2O2-induced DNA damage in single cell gel electrophoresis assay (comet assay) by castasterone isolated from leaves of centella asiatica

  AUTHORS: Nishi Sondhi, Renu Bhardwaj, Satwinderjeet Kaur, Madhu Chandel, Neeraj Kumar, Bikram Singh

  KEYWORDS: Brassinosteroids; Castasterone; Comet Assay; Hydrogen Peroxide

  JOURNAL NAME: Health, Vol.2 No.6, June 30, 2010

  ABSTRACT: Brassinosteroids (BRs) are a large group of polyhydroxy steroids, which regulate numerous aspects of plant growth and development, including stem elongation, leaf bending, tracheary element differentiation, stress protection and photomorphogenesis. Recent studies indicate antigenotoxic and anticancerous activities of these compounds. The role of natural BRs in H2O2 (hydrogen peroxide) -induced DNA damage in human lymphocytes is still unknown. The present study reports the presence of Castasterone from leaves of Centella asiatica, an important medicinal herb commonly used as a memory enhancer and immunomodulator. CA50 fraction isolated from Centella asiatica was characterized as Castasterone by electrospray ionization mass spectral data with standard Castasterone. An attempt has been made to study antigenotoxic activity of the isolated Castasterone against H2O2 -induced DNA damage in human blood lymphocytes using Single cell gel electrophoresis assay (Comet Assay). Castasterone at 10–9 M concentration proved to be effective in diminishing the DNA damage by 89.42 %.