TITLE:
Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients
AUTHORS:
Mai Mohamed Abdelwahab, Huda Fathy Ebian, Taiseer Refat Ibrahim, Mohammad Samir Badr, Mohamed El-Bakry Lashin, Mahmoud Abdou Yassin, Adel Mohamed Ismail, Ahmed Ali Obaya
KEYWORDS:
Serous Ovarian Carcinoma, Serum, Immunohistochemistry, Galectin-1, Overall Survival
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.9 No.7,
July
5,
2019
ABSTRACT: Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type
with poor prognosis due to early metastasis and first presentation with
advanced stage. In this work, we investigated serum level of Galactin-1 (Gal-1) and its tissue
immunohistochemical expression in SOC patients at different stages trying to
find out its significance as a diagnostic and prognostic marker. Patients and methods: The
study included 95 females I-Control group: Twenty five healthy females; II-Patients group:
Seventy females diagnosed as SOC at different stages; Stage I: 8 cases, Stage II:
12 cases, Stage III: 32 cases and Stage VI:18 cases. Serum Galectin-1 and
CA-125 were measured by ELIZA and tissue Galectin-1 was assessed by immunohistochemistry.
All patients were followed for up to 3 years after surgery. Results: Serum Gal-1 and CA-125 levels were significantly
higehr in SOC patients compared to controls (p 0.001). We found a direct
positive statistically significant correlation between serum Gal-1 and CA125
levels (p 0.001). Serum Gal-1 at cut
off value > 135
ng/ml was superior to CA-125 a cut off value > 49 u/ml with sensitivity,
specificity of 100%, vs 88.57, 96% for CA-125. Serum Gal-1 was significantly
associated with tumor stage (p 0.001). Immunohistochemistry
showed that patients with strong Gal-1 expression had higher serum level (p = 0.002). Stromal and tumor Gal-1 expression were significantly
correlated with tumor grade (p 0.001) and stage (p = 0.001). Serum Gal-1, CA-125 and IHC Gal-1 expression
were associated with poor survival (p 0.001,
p = 0.009 and p = 0.002) respectively. Conclusion: Serum Gal-1 and its
tissue IHC expression are useful diagnostic and prognostic markers for SOC
patients.