TITLE:
HPV18 E6 and E7 Intratumour Heterogeneity in Esophageal Cancer
AUTHORS:
Sara Khodahemmati, Maliha Gaffar, Jintao Li, Yangjunqi Wang, Xiaoli Wang, Zhixiang Zhou, Yi Zeng
KEYWORDS:
Esophageal Cancer, Human Papillomavirus, HPV 18 E6 and E7, Single Cell Cloning, Intra-Tumor Heterogeneity
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.10 No.5,
May
9,
2019
ABSTRACT: The
development of esophageal cancer accompanied by the presence of human
papillomavirus (HPV) DNA into the host genome. By evaluating the expression of
this virus for tumor cell origin and also their cell grows and migrations, we
examined esophageal cancer clonality in the context of intra-tumor
heterogeneity. In this research, we have checked the expression of HPV18 E6 and
E7 in different single cell clones by the manual cell picking method in the HPV
positive esophageal cancer (EC109), EC109 cell line used as a negative control,
and Hela cell line used as the positive control. Quantitative real-time PCR (QRT-PCR) was run to detect the expression levels of
HPV E6 and E7, Cell Counting Kit-8 (CCK-8) assay was used to examine cell
proliferation, invasion assays performed using Costar chambers and wounding
assay to study cell migrations in vitro.
We investigated the intra-tumor heterogeneity of HPV E6 and E7 in esophageal
cancer and the evaluation of the growth and migrations at the clonal level,
using 10 single cell clones. In particular clones, C7 & C10 displayed a highly variable expression in both
HPV E6 and E7 and weak in four clones (C1, C3, C4, and C9) consequently, the
cell invasion,
proliferation, and migration increase with increasing the level of HPV
expression and inverse. In
conclusion, the resulting based on single cell cloning showed the relationship
between HPV and cell growth and migration in esophageal cancer. Future study in
HPV DNA integration needed to explore the mains specific integration site of
HPV DNA in esophageal cancer and molecular monitoring of the HPV for future prevention researches and
also effective therapeutic strategies.