TITLE:
Antioxidant, Cytotoxic, Thrombolytic and Antimicrobial Activity of Zanthoxylum rhetsa Root Bark with Two Isolated Quinolone Alkaloids
AUTHORS:
Fatema Tuz Zohora, Sheikh Nazrul Islam, Shibbir Ahmed Khan, Choudhury Mahmood Hasan, Monira Ahsan
KEYWORDS:
Zanthoxylum rhetsa, Antioxidant, Thrombolytic, Antimicrobial, 8-Methoxy-n-Methylflindersine, Zanthodioline
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.10 No.3,
March
22,
2019
ABSTRACT:
The background of this study was to investigate the
antioxidant, cytotoxic, thrombolytic and antimicrobial activity of the
petroleum ether (PE), carbon tetrachloride (CTC), chloroform (CF) and aqueous
(AQ) soluble fractions of crude methanolic Zanthoxylum
rhetsa root bark with two isolated quinolone alkaloids, 8-methoxy-n-methylflindersine
(1) and zanthodioline (2). Structures were characterized by 1D
NMR analyses. Antioxidant activity was assessed by using DPPH assay and
antimicrobial activity was screened by disc diffusion method. An in vitro thrombolytic model was used to
evaluate the clot lysis effect of different extracts of root bark of Z. rhetsa along with streptokinase as a
positive control and distilled water as a negative control and the cytotoxic
activity of different extracts of Z. rhetsa root bark was evaluated by Brine
Shrimp Lethality Bioassay. AQ fraction exhibited strongest antioxidant,
cytotoxic and thrombolytic activity among four fractions. The CTC and CF
soluble fractions exhibited significant antioxidant, cytotoxic and thrombolytic
activity. CTC and AQ fractions gave highest anti-bacterial activity against Vibrio cholera and Klebsiella pneumonia respectively. Compound 1 showed
significant activity at a concentration of 100 μgm/disc against Sarcina lutea, Staphylococcus aureus and Salmonella paratyphi-A, Shigella dysenteriae, Shigella boydii and Shigella sonnei with high antioxidant activity. The antioxidant, thrombolytic and antimicrobial
activity of 8-methoxy-n-methylflindersine and zanthodioline are the first
record from root bark of this plant.