TITLE:
Volatile Compounds Analysis of Tunisian Propolis and Its Antifungal Activity
AUTHORS:
Ayari Jihene, Ines Jabri Karoui, Ayed Ameni, Majdi Hammami, Manef Abderrabba
KEYWORDS:
Propolis, Headspace/GC-MS, α-Pinene, PCA, Antifungal Activity
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.6 No.6,
June
28,
2018
ABSTRACT: In this study, four samples of Tunisian propolis
were analyzed for their volatile compounds. Static Headspace technique coupled
with gas chromatography-mass spectrometry (GC/MS) was used for the volatile
profile characterization. Statistical investigation of the samples was made
applying hierarchical clustering method, K-Means cluster and principal component
analysis. Results showed that 47, 36, 30 and 24 different compounds were
identified, respectively, in the samples from Zouarine, Zelligua, Bizerte and Beni Khalled. Propolis volatiles were dominated
by monoterpene hydrocarbons. α-pinene
was the major compound representing 81.14%, 82.67% and 90.74%, respectively, of
the total propolis volatiles collected from Zelligua, Beni Khalled and Zouarine
and only 45.22% of the sample from Bizerte which had a very different
composition. The in vitro antifungal
activity of the volatiles from all samples against Candida albicans was
also assayed and reported.