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http://dx.doi.org/10.1590/S0100-204X2001001200015

has been cited by the following article:

• TITLE: Seed Quality Evaluation by Tetrazolium Staining of Parkia multijuga Benth

  AUTHORS: Marcelle Auday Costa, Elizabeth Santos Cordeiro Shimizu, Noemi Vianna Martins Leão, Hugo Alves Pinheiro

  KEYWORDS: Forest Seeds, Fava-Arara-Tucupi, Viability, Amazon

  JOURNAL NAME: Agricultural Sciences, Vol.9 No.5, May 31, 2018

  ABSTRACT: The purpose of this study was to develop a staining pattern through tetrazolium test to evaluate seed viability of Parkia multijuga Benth (fava-arara-tucupi). The seeds were scarified, soaked into water and distilled for 48 hours for tegument removal and then immersed in a 0.05%; 0.1%; and 0.3% tetrazolium solution for 6; 18 and 24 hours at 35°C, in the dark. The evaluation was performed on the basis of stain intensity and aspect of the tissues. Based on color patterns, the seed images were classified in eight viability classes. Those results were compared with the germination test in a laboratory. The treatment in concentration of 0.1% for 6 hours was considered adequate for the specie, considering the adequate staining and time for analysis.