TITLE:
Variability of Deltamethrin-Resistant Metarhizium anisopliae Aggressive Strains Group to a Population of the Cossid Moth from Eucalyptus nitens
AUTHORS:
Pedro Romón, Hardus Hatting, Arturo Goldarazena, Juan Carlos Iturrondobeitia
KEYWORDS:
Coryphodema tristis, Metarhizium anisopliae, Assays, Compatibility with Deltamethrin, RAPDs
JOURNAL NAME:
Open Journal of Forestry,
Vol.8 No.2,
April
20,
2018
ABSTRACT: Random amplified
polymorphic DNA-polymerase chain reaction
(RAPD-PCR) was used to examine the genetic
variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates
tightly clustered into one or the other of two groups that diverged at 12%.
Results suggested that certain genotypes of the fungus, that grouped together,
were able to infect moth larvae while others did not. A fragment of 760 bp,
which presents high homology with a host-adaptation related protein coding
gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial
growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates,
including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all
the larvae after seven days whereas fungal and mixed treatments respectively
reached the same mortality after 28 and 21 days.