Mitra, R., Cuesta-Alonso, E., Wayadande, A., Talley, J., Gilliland, S. and Fletcher, J. (2009) Effect of Route of Introduction and Host Cultivar on the Colonization, Internalization, and Movement of the Human Pathogen Escherichia coli O157: H7 in Spinach. Journal of Food Protection, 72, 1521-1530.
https://doi.org/10.4315/0362-028X-72.7.1521

has been cited by the following article:

• TITLE: Evaluation of Organic Acid-Based Sanitizers for Reduction of Escherichia coli O157:H7 during Flume-Washing of Organic Leafy Greens

  AUTHORS: Pushpinder Kaur Litt, Justin Brooks, Divya Jaroni

  KEYWORDS: Organic Sanitizers, Escherichia coli O157:H7, Organic Leafy Greens, Antimicrobials

  JOURNAL NAME: Food and Nutrition Sciences, Vol.8 No.10, October 24, 2017

  ABSTRACT: Antimicrobial efficacy of three novel organic sanitizers, CHICO WashTM, C8C10 and CG100, was evaluated for the reduction of Escherichia coli O157:H7 during flume-washing of organic leafy greens. Organic formulations at various concentrations: CHICO (C3H8I0C3O7) WashTM (1:20 ratio) and C8C10 and CG100 (0.2 and 0.4%), along with the controls: hydrogen-peroxide and water, were used for washing organic baby and mature spinach and romaine and iceberg lettuce. Leafy greens were inoculated with a 2-strain cocktail of E. coli O157:H7 (6 logs CFU/mL) and washed in each treatment for 1 or 2 minutes. The treated leafy greens were stored at 4°C and surviving pathogen populations determined on days 0, 1, and 3 of storage. Organic sanitizers, for both treatment times, significantly (P E. coli O157:H7 on all the leafy greens during storage. Highest reduction (3.4 logs CFU/g) was observed after treatment with CG100 (0.4%) in romaine lettuce, while CHICO WashTM showed greater than 2 logs CFU/g reduction on all the leafy greens, by day 3. This study demonstrates the potential application of organic sanitizers in flume-washing of organic leafy greens for the reduction of E. coli O157:H7.