TITLE:
Activity of the Enzyme Gamma-Glutamyl Transferase (GGT) as a Prognostic Tool for Heart Failures
AUTHORS:
Susan J. Ali, Ayoub A. Bazzaz, Abdulwadood I. Arif
KEYWORDS:
Purification, GGT-Enzyme, Heart Failure, Gel-Electrophoresis, Iso-Enzymes-I
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.8 No.9,
September
30,
2017
ABSTRACT: Heart
failure (HF) incidence could cause further complications to other body organs,
which might sometimes be fatal, and is
accompanied by various biochemical alterations i.e. enzymatic changes. The objective of this study was to measure
the activity of gamma glutamyl transferase (GGT) as an early diagnostic
indicator for HF patients; and to isolate the iso-enzymes for the purpose of
finding the Michaelis-Menten constant (Km) and the maximum velocity Vmax of
each iso-enzymes which enable follow up the development of HF disease. Samples
of blood sera were collected from 120 patients of both genders (70 males and 50
female, aged 30 - 38 years old). Partial purification of iso-enzyme GGT was performed by
precipitation, gel filtration, and ion exchange of the two iso-enzyme (I and
II). The purity of the enzyme was confirmed by using Sodium dodecyl
sulfate-polyacryl amide gel electrophoresis (SDS-PAGE) into two clear bands.
The results were compared with other 80 samples of healthy volunteers whose
ages ranged between 25 - 78 years old, used as control. There has been a significant increase (p ≤ 0.01) in the activity of the enzyme GGT in the heart failure patient
(66.9 ± 1.7 IU/L) in comparison with control (12.07 ± 0.60
IU/L). It is concluded that measurements of the iso-enzyme GGT could well
benefit as a clear indicator criteria in prognosis of heart failure.