TITLE:
In Vitro Evaluation of Anticancer Drugs with Kinetic and Static Alternating Cell Culture System
AUTHORS:
Yongxin Zhang, Ying Wang, Zhenying Wang, Farhang Farhangfar, Monica Zimmerman
KEYWORDS:
Anticancer Drug Evaluation, Cell Culture, Bioreactor
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.8 No.9,
September
27,
2017
ABSTRACT: Variable bioreactors have been developed for the
evaluation of anti-cancer drug efficacy.
The Kinetic and Static Alternating Cell Culture System (KSACCS) combines
the advantages of kinetic bioreactors and static cultures to improve cell
growth by providing adequate metabolic support while minimizing shear-stress.
In the current studies, the KSACCS in the ZYX Bioreactor could significantly increase
the sensitivity of lung cancer cells (PLS008) and leukemia cells (HL60) to
anticancer drugs Cisplatin and 5-FU by accelerating the apoptosis of cancer
cells. It was also shown that excessive agitation of the cells could lead to
severe cell damage, which resulted in a diminished sensitivity of anticancer
drug evaluation, and co-culture systems tend to reduce the sensitivity of
anticancer drug evaluation although it might better mimic in vivo conditions.