TITLE:
Bacterial Community Structure and Diversity of Closely Located Coastal Areas
AUTHORS:
Md. Nurul Haider, Masahiko Nishimura, Kazuhiro Kogure
KEYWORDS:
Coastal Bacteria, Community Structure, Diversity, High-Throughput Sequencing, Roche 454
JOURNAL NAME:
Open Journal of Marine Science,
Vol.6 No.3,
July
28,
2016
ABSTRACT: Bacterial community
structure and diversity of two closely located stations are usually considered
similar which can be verified by more intensive investigations using relatively
large amount of datasets from the next generation sequencer. This study was
conducted to assess the bacterial community structure and diversity between two
closely located coastal stations, the port side and the sea side of the Oarai,
Ibaraki, Japan from March 2013 to July 2014 using 454 GS Junior sequencer.Twostations
underwent similar changes in physicochemical properties but the community
structure and diversity was different. The Proteobacteria (the class
Alphaproteobacteria, followed by the Gammaproteobacteria) and the Bacteroidetes
(the class Flavobacteriia) were two abundant phyla in both the stations. But,
the Flavobacteriia was more abundant in the port side, contributed about 26% to
48%, compared to the sea side (about 12% to 39%). Conversely, the relative
abundance of the Gammaproteobacteria was higher on the sea side, about 10% to
17%, compared to the port side (about 4% to 12%). Among others, the phyla
Cyanobacteria, Deferribacteres, Verrucomicrobia and the class Betaproteobacteria
were also relatively abundant at thesea
side. Because of their dominancy, the class Flavobacteriia and
Alphaproteobacteria were furtheranalysed
at a lower phylogenetic level and marked differences were observed between the
stations.Bacterial biodiversity
in terms of the species richness (Chao index) and evenness (inverse Simpson) indicated
higher patterns of diversity in the sea side area compared to the port side.
Non-metricMultidimensional
Scaling fitting with the environmental features (metaMDS), redundancy analysis
(RDA) and Bray-Curtis clustering analysis also showed marked differences in
bacterialcommunitystructure and diversity between the
stations. However, some OTUs were commonly found in both the stations in all
the sampling periods.So, the
bacterial community structure and diversity of the coastal areas are
distinguishable even between two closely located sampling points.