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Temple, N.J. (2000) Antioxidants and Disease: More Questions than Answers. Nutrition Research, 20, 449-459.

has been cited by the following article:

  • TITLE: Effect of Induced Mutation on Antioxidant Activity in Ocimum basilicum Linn

    AUTHORS: Isaac Kojo Asante, Kofi Annan, Matthew Kweku Essilfie, Valentine Tater

    KEYWORDS: Ocimum basilicum, Nonirradiated, Irradiated, Antioxidant Activity, Mutant, 1, 1-Diphenyl-2-picryl-hydrazyl

    JOURNAL NAME: Natural Science, Vol.8 No.4, April 18, 2016

    ABSTRACT: Five batches from the same stock of seeds of Ocimum basilicum were irradiated at 5, 10, 15, 20 and 25 Gy, respectively using 60C source. Methanolic leaf extracts of these samples and a control were evaluated for their antioxidant activity by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method using M2 plants. All the methanolic extracts showed antioxidant activity. The IC50 of the methanolic extracts of the six different treatments, control, 5 Gy, 10 Gy, 15 Gy, 20 Gy and 25 Gy, showed antioxidant activity with IC50 values of 100, 90, 86, 61, 71 and 70 μg/ml, respectively. Three individual mutants, M-15-5, M-20-6 and M-15-4, had IC50 values of 26, 30 and 40 μg/ml, respectively. These mutants were from the 15 Gy and 20 Gy treatments. From the results, it is confirmed that induced mutation can be employed to create variation in the levels of free radical scavenging activity in O. basilicum and can therefore serve as a tool for breeding for high levels of antioxidant activity in O. basilicum.