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http://dx.doi.org/10.1097/01.TP.0000045055.63901.A9

has been cited by the following article:

• TITLE: Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models

  AUTHORS: Titiek Ernawati, Gatut Suhendro, I Ketut Sudiana, Suhartono Taat Putra, Harjanto JM,   Sunarjo, Agus Turchan, Fedik Abdul Rantam

  KEYWORDS: Hypoxic Preconditioning, Transplantation, Bone Marrow-Mesenchymal Stem Cells, BM-MSCs, Glaucoma, Neuroprotective

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.9 No.4, March 30, 2016

  ABSTRACT: This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p