Article citationsMore>>
Xiong, D.H., Liu, X.G., Guo, Y.F., Tan, L.J., Wang, L., Sha, B.Y., Tang, Z.H., Pan, F., Yang, T.L., Chen, X.D., Lei, S.F., Yerges, L.M., Zhu, X.Z., Wheeler, V.W., Patrick, A.L., Bunker, C.H., Guo, Y., Yan, H., Pei, Y.F., Zhang, Y.P., Levy, S., Papasian, C.J., Xiao, P., Lundberg, Y.W., Recker, R.R., Liu, Y.Z., Liu, Y.J., Zmuda, J.M. and Deng, H.W. (2009) Genome-Wide Association and Follow-Up Replication Studies Identified ADAMTS18 and TGFBR3 as Bone Mass Candidate Genes in Different Ethnic Groups. The American Journal of Human Genetics, 84, 388-398.
http://dx.doi.org/10.1016/j.ajhg.2009.01.025
has been cited by the following article:
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TITLE:
Arg-Ser-775, 792 and 823 in Spacer Region of ADAMTS-18 Is Critical for Thrombin Cleavage
AUTHORS:
Jian Tang, Wei Huang, Ning Shen, Tao Hong, Suying Dang, Wei Zhang
KEYWORDS:
ADAMTS-18, Thrombin, Cleavage, Mass Spectrum
JOURNAL NAME:
Health,
Vol.6 No.18,
October
16,
2014
ABSTRACT: Cleavage of ADAMTS-18
by thrombin represents a new mechanism of platelet thrombus clearance via the
release of active ~45-kDa C-terminal fragments that induces oxidative platelet
fragmentation. The exact cleavage sites remain unclear, but Arg (R)775/Ser
(S)776 in
spacer region of ADAMTS-18 has been shown to be one of the cleavage sites of
thrombin. Here, we demonstrate that R792/S793 and R823/S824 are also thrombin cleavage sites by sequence analysis, amino acid mutation and
mass spectrometry assay. All these cleavage sites are thrombin-specific and
insensitive to other enzymes tested (e.g. cathepsin D or trypsin). Simultaneous
mutation of R775, 792, 823 to S775, 792, 823 in
ADAMTS-18 completely abrogated the cleavage by thrombin and the generation of
active C-terminal 45-kDa fragments. Together with previous study, a total of
three thrombin-specific cleavage sites have been identified in spacer region of
ADAMTS-18.
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