TITLE:
A New Diterpene Extracted from Daphne Mucronata, Effects on Human K562 and CCRF-CEM Cell Lines
AUTHORS:
Mehdi Hedayati, Razieh Yazdanparast, Marjan Zarif Yeganeh, Laleh Hoghooghi Rad, Fereidoun Azizi
KEYWORDS:
TNF-α, Leukemia, Daphne Mucronata, K562, CCRF-CEM
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.2 No.1,
March
15,
2011
ABSTRACT: Background: Daphne Mucronata extract has a decreasing effect on the size of breast adenocarcinoma in rats. So in this study, the effect of Daphne Mucronata purified diterpene were investigated on co culture of human monocytes and two human leukemia cell lines (K562, CCRF-CEM). Materials and Methods: Each cell line mono-layer culture, in log phase growth, was treated with 10 to 160 μL of the extract (1 g/ml leave powder) and purified compound (0.94 nM). For a comparative study, Taxol (5 to 40 μM) was used in the presence and absence of LPS. Human monocytes were isolated by adhesion method. TNF-α in cultured media were measured by sensitive biotin-streptoavidin ELISA method. Results: Fifty percent of growth inhibition was shown by 160 μL (1:100 dilution, 0.5 g of the powdered leaves/ml) of the extract and 0.94 nM of the purified component, and there was more inhibition in K562 cells (P