This present study attempts to examine the effect of sanctioning systems on trust and cooperative behaviors in a public goods dilemma by using an experiment paradigm of “removing the sanctions”. Three experimental conditions, no-sanction, low sanction and high sanction, were used. 96 undergraduate students participated in the experiment, and the participants were randomly assigned to 24 groups with four persons each group. The results show that participants exhibited higher level of trust in others in two sanction conditions than in no-sanction condition. However when the sanctioning system was removed, the level of trust in others decreased significantly. In a similar way, the sanctioning system undermined cooperation and seemed particularly true when initial sanction was high.