Determination of Magnesium in whole Blood and Serum of Ischemic Heart Disease(IHD) Patients by Flame Atomic Absorption Spectrometry
Hassan T. Abdulsahib
DOI: 10.4236/ajac.2011.28117   PDF    HTML     5,881 Downloads   9,853 Views   Citations


Flame atomic absorption spectrometric determination of magnesium in whole blood and serum of ischemic heart disease patients and control with different ages and sex was proposed. The limiting interfering phosphate/magnesium ratio have been estimates.2%w/v AlCl3.6H2O was found to be very influential in removing phosphate interference effects. The detection limit was 0.065 μg/mL .Magnesium added to blood and serum sample, and carried through this method may be recovered completely (96% - 100%) recovery percentage. The suggested method is simple, fast and selective. The statistical analysis of magnesium levels in blood and serum showed that blood and serum magnesium levels in patients were lower than magnesium contents of control group. Magnesium levels in blood and serum of males were significantly lower than females and the magnesium levels were age independent. These findings indicates that there was an association between blood and serum magnesium deficiency which can induce an entire array of path physiological phenomena known to be important in ischemic heart disease.

Share and Cite:

H. Abdulsahib, "Determination of Magnesium in whole Blood and Serum of Ischemic Heart Disease(IHD) Patients by Flame Atomic Absorption Spectrometry," American Journal of Analytical Chemistry, Vol. 2 No. 8, 2011, pp. 996-1002. doi: 10.4236/ajac.2011.28117.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] National Research Counsil, “Diet and Health,” National Academy Press, Washington DC, 1989.
[2] S. Chakraborti, T. Chakraborti, M. Mandal, A. Mandal, S. Das and S. Ghosh, “Protective Role of Magnesium in Cardiovascular Disease: A Reiew,” Molecular and Cellular Biochemistry, Vol. 238, No. 1-2, 2002, pp. 163-179. doi:10.1023/A:1019998702946
[3] N. E. Saris, E. Mervaala, H. Karppanen, J. A. Khawaja and A. Lewenstam, “Magnesium. An Update on Physiological, Clinical and Analytical Aspects,” Analytica Chimica Acta, Vol. 294, No. 1-2, 2000, pp. 1-26.
[4] G. E. Burch and T. D. Giles, “The Importance of Magnesium Deficiency in Cardiovascular Disease,” American Heart Journal, Vol. 94, No. 5, 1977, pp. 649-657. doi:10.1016/S0002-8703(77)80137-3
[5] P. C. Elwood and W. H. Beasley, “Myocardial Magnesium and Ischaemic Heart Disease,” Artery, Vol. 9, 1981, pp. 200-204.
[6] I. Bersohn, “Atherosclerosis and Coronary Heart Disease: Possible Incrimination of Magnesium Deficiency in Their Promotion,” Medical Procedures, Vol. 4, 1958, p. 62.
[7] M. S. Seelig, “Possible Roles of Magnesium in Disorders of the Aged,” In: R. W. Gelson and F. M. Sinex, Eds., “Intervention in the Aging Process. Part A: Quantitation, Epidemiology,” Clinical Research, AR Liss, Inc, New York, 1983, pp. 279-305.
[8] J. G. Wang, “Analysis of Clinical Biochemistry,” Hunan Scientific & Technological Publishing Company, Changsha, 1980.
[9] “Analytical Methods for Flame Spectroscopy,” Varian Techtron Pty. Limited, Mulgrave, 1979.
[10] P. W. Sunderman and F. Boerner, “Normal Values in Clinical Medicine,” Saunders, Philadelphia, 1950.
[11] W. S. Spector, “Handbook of Biological Data,” Wright Air Development Center, New York, 1956.
[12] R. Elin, “Laboratory Tests for Assessment of Magnesium Status in Humans,” Magnesium and Trace Elements, Vol. 10, 1991-1992, pp. 172-181.
[13] E. B. Flink, J. E. Brick and S. R .Shane, “Alterations of Long-Chain Free Fatty Acid and Magnesium Concentrations in Acute Myocardial Infarction,” Archives of Internal Medicine, Vol. 141, No. 4, 1981, pp. 441-443. doi:10.1001/archinte.1981.00340040037014
[14] E. B. Flink, S. R. Shane, R. R. Scobbo, N. G. Blechschmidt and P. McDowell, “Relationship of Free Fatty Acids and Magnesium in Ethanol Withdrawal in Dogs,” Metabolism, Vol. 28, No. 8, 1979, pp. 858-865. doi:10.1016/0026-0495(79)90213-0
[15] D. A. Elliott and M. A. Rizack, The Journal of Biological Chemistry, Vol. 249, 1973, pp. 3985-3990.
[16] H. Ebel and T. Günther, “Role of Magnesium in Cardiac Disease,” Journal of Clinical Chemistry & Clinical Biochemistry, Vol. 21, 1983, pp. 249-265
[17] M. Haigney, B. Silver, E. Tanglao, H. Silverman, D. Hill, E. Shapiro, G. Gerstenblith and S. Schulman, “Nonivasive Measurement of Tissue Magnesium and Correlation with Cardiac Levels,” Circulation, Vol. 92, 1995, pp. 2190-2197.
[18] H. Rasmussen, P. Aurup, S. Hojberg, K. Jensen and P. McNair, “Magnesium and Acute Myocardial Infarction,” Archives of Internal Medicine, Vol. 146, No. 5, 1986, pp. 872-874. doi:10.1001/archinte.1986.00360170068010
[19] M. R. England, G. Gordon, M. Salem and B. Chernow, “Magnesium Administration and Dysrhythmias after Cardiac Surgery. A Placebo-Controlled, Double-Blind, Randomized Trial,” JAMA, Vol. 268, No. 17, 1992, pp. 2395-2402. doi:10.1001/jama.1992.03490170067027
[20] R. S. Parsons, T. C. Butler and E. P. Sellars, “Hardness of Local Water Upplies and Mortality from Cardiovascular Disease,” Lancet, Vol. 278, No. 7195, 1961, p. 213. doi:10.1016/S0140-6736(61)90505-0
[21] P. M. Savenkov, A. K. Martynov, G. I. Kertsman, O. B. Bobrooskaya and A. D. Pogorelsky, “The Use of Magnesium Composition in Patients with Atherosclerosis of Vessels of the Heart, Brain, and Lower Extremities,” Kardiologia, Vol. 11, 1971, p. 85.
[22] R. S. Parsons, T. Butler and E. P. Sellars, “The Treatment of Coronary Artery Disease,” Med. Proc., Vol. 5, 1959, p. 487.
[23] K. H. Hackethal and Z. Den, “Aussprache Vortragen I and II,” Chirurgie, Vol. 35, 1951, p. 270.
[24] H. G. Heinrich, “Prophylaxe und Therapie Thrombotischer Zustande Mit Magnesium,” Zschs Gesell Inn Medicine, Vol. 12, 1957, p. 777.
[25] B. Schitzler, “Thromboseprophylaxe Mit Magnesium,” Munchen Medical Wochschr, Vol. 99, 1957, p. 81.
[26] J. Durlach, “Pilule et Thrombose (des Plaquettes,des Estrogens et du Magnesium),” Reviews Franclin Endocninology Clinical, Vol. 11, No. 45, 1970.
[27] N. G. Ardlie, E. E. Nisizawa and M. Guccione, “Effect of Ca and Mg on Platelet Function,” Federation Procedures, Vol. 29, 1970, p. 423.
[28] R. S. Tonks, “Haematology-Magnesium, Adenosine Diphosphate and Blood Platelets,” Nature, Vol. 210, 1966, p. 106.
[29] M. S. Seelig, Magnesium Bull, Vol. 3, 1981, pp. 26-47.
[30] R. Fehlinger, L. Franke, E. Glatzel, E. Meyer, M. Michalik, S. M.Rapoport, M. Rüstow, C. Schulz and G. Schumann, Magnesium Bull, Vol. 3, 1981, pp. 298-306.
[31] F. Liao, A. R. Folsom, F. L. Brancati, “Is Low Magnesium Concentration a Risk Factor for Coronary Heart Disease? The Atherosclerosis Risk in Communities (ARIC) Study,” American Heart Journal, Vol. 136, No. 3, 1998, pp. 480-490. doi:10.1016/S0002-8703(98)70224-8
[32] P. S. Gartside and C. J. Glueck, “The Important Role of Modifiable Diatary and Behaivioral Characteristic in the Causation and Prevention of Coronary Heart Disease Hospitalization and Mortality: The Prospective NHANES Followup Study,” Journal of the American College of Nutrition, Vol. 14, 1995, pp. 71-79.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.