Human embryonic stem cell lines with ccr5-del32 allele conferring resistance to HIV


A 32bp deletion in the chemokine receptor 5 (CCR5) gene (CMKBR5) was shown to be linked to HIV resistance. Bone marrow transplantation from the homozygous CCR5-del32 donor to a CDC Stage 2 HIV-positive recipient was demonstrated to confer a HIV resistance, resulting in discontinuation of antiretroviral therapy. In search for an unlimited source of CCR5-del32 cells for transplantation purposes, we tested 137 human embryonic stem cell (hESC) lines from the Reproductive Genetics Institute’s hESC lines collection, and report here the finding of 12 hESC lines with the CCR5-del32 allele, one of which represents a unique partenogenetic ESC line containing two copies of this deletion and may be studied for utility in stem cell transplantation treatment of HIV.

Share and Cite:

Pomerantseva, E. , Kukharenko, V. , Goodman, A. , Verlinsky, O. , Rechitsky, S. and Kuliev, A. (2011) Human embryonic stem cell lines with ccr5-del32 allele conferring resistance to HIV. Stem Cell Discovery, 1, 67-70. doi: 10.4236/scd.2011.13007.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Hutter, G., Nowak, D., Mossner, M., Ganepola, S., Mussig, A., Allers, K., Schneider, T., Hofmann, J., Kucherer, C., Blau, O., Blau, I.W., Hofmann, W.K. and Thiel, E. (2009) Long-term control of HIV by CCR5 Delta32/Delta32 stem-cell transplantation. New England Journal of Medicine, 360, 692-698.
[2] Liu, R., Paxton, W.A., Choe S., Ceradini, D., Martin, S.R., Horuk, R., MacDonald, M.E., Stuhlmann, H., Koup, R.A. and Landau, N.R. (1996) Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell, 86, 367-377. doi:10.1016/S0092-8674(00)80110-5
[3] Beatty, P.G., Boucher, K.M., Mori, M. and Milford, E.L. (2000) Probability of finding HLA-mismatched related or unrelated marrow or cord blood donors. Human Immunology, 61, 834-840. zdoi:10.1016/S0198-8859(00)00138-5
[4] Majhail, N.S., Brunstein, C.G., Tomblyn, M., Thomas, A.J., Miller, J.S., Arora, M., Kaufman, D.S., Burns, L.J., Slungaard, A, McGlave, P.B., Wagner, J.E., Weisdorf, D.J. (2008) Reduced-intensity allogeneic transplant in patients older than 55 years: Unrelated umbilical cord blood is safe and effective for patients without a matched related donor. Biology of Blood and Marrow Transplantation, 14, 282-289. doi:10.1016/j.bbmt.2007.12.488
[5] Kleen, T.O., Kadereit, S., Fanning, L.R., Jaroscak, J., Fu, P., Meyerson, H.J., Kulchycki, L., Slivka, L.F., Kozik, M., Tary-Lehmann, M. and Laughlin, M.J. (2005) Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation. Transplantation, 80, 1316-1322. doi:10.1097/
[6] Verlinsky, Y., Strelchenko, N., Kukharenko, V., Shkumatov, A., Rechitsky, S., Verlinsky, O. and Kuliev, A. (2006) Repository of human embryonic stem cell lines and development of individual specific lines using stembrid technology. Reproductive BioMedicine Online, 13, 547-550.
[7] Verlinsky, Y., Strelchenko, N., Kukharenko, V., Shkumatov, A., Rechitsky, S., Verlinsky, O. and Kuliev, A. (2009) Isolation of human embryonic stem cells from various stages of the human embryo. In: Lakshmipathy et al. Ed, Emerging Technology Platforms for Stem Cells, Wiley, Hoboken, 19-27. doi:10.1016/S1472-6483(10)60643-8
[8] Robertson, N.J., Brook, F.A., Gardner, R.L., Cobbold, S.P., Waldmann, H. and Fairchild, P.J. (2007) Embryonic stem cell-derived tissues are immunogenic but their inherent immune privilege promotes the induction of tolerance. Proceeding of the National Academy Science of the USA, 104, 20920-20925.
[9] Wu, D.C., Boyd, A.S. and Wood, K.J. (2008) Embryonic stem cells and their differentiated derivatives have a fragile immune privilege but still represent novel targets of immune attack. Stem Cells, 26, 1939-1950. doi:10.1634/stemcells.2008-0078
[10] Verlinsky, Y. and Kuliev, A. (2006) Practical preimplantation genetic diagnosis. Springer, London and New York, 198.
[11] Martinson, J.J., Chapman, N.H., Rees, D.C., Liu, Y.T. and Clegg, J.B. (1997) Global distribution of the CCR5 gene 32-basepair deletion. Nature Genetics, 16, 100-103. doi:10.1038/ng0597-100
[12] Taylor, C. Bolton, E., Procock, S., Sharples, L., Pederson, R. and Bradley, J. (2005) Banking of human embryonic stem cells: Estimating the number of donor cell lines needed for HLA matching. Lancet, 366, 2019-2025. doi:10.1016/S0140-6736(05)67813-0
[13] Pryzhkova, M.V., Peters, A. and Zambidis, E.T. (2010) Erythropoietic differentiation of a human embryonic stem cell line harbouring the sickle cell anemia mutation. Reproductive BioMedicine Online, 21, 196-205. doi:10.1016/j.rbmo.2010.04.017

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.