Introgressive Hybrids of Arisaema sikokianum and A. tosaense (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons


Morphologically putative introgressive hybrids of Arisaema sikokianum Franch. et Sav. and A. tosaense Makino were newly found in Kochi and Tokushima Prefectures in Japan. All the individuals have the same morphological characteristics as A. tosaense excluding a purple spathe. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that these putative introgressive hybrids have the same pattern as A. tosaense. Moreover, the sequences of chloroplast DNA (cpDNA) of the putative introgressive hybrids were identical to A. sikokianum from Kochi Prefecture and A. tosaense from Tokushima Prefecture. The results suggest that the plants are introgressive hybrids of A. sikokianum and A. tosaense and that they have highly exchanged genes with A. tosaense.

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Hayakawa, H. , Hamachi, H. , Matsuyama, K. , Muramatsu, Y. , Minamiya, Y. , Ito, K. , Yokoyama, J. and Fukuda, T. (2011) Introgressive Hybrids of Arisaema sikokianum and A. tosaense (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons. American Journal of Plant Sciences, 2, 303-307. doi: 10.4236/ajps.2011.23034.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] J. F. Wendel and J. J. Doyle, “Phylogenetic Incongruence: Window into Genome History and Molecular Evolution,” In: D. E. Soltis, P. S. Soltis and J. J. Doyle, Eds., Mo- lecular Systematics of Plants II and Sequencing, Kluwer Academic Publishers, Norwell, 1998, pp. 265-296.
[2] H. Ohashi, “Araceae,” In: Y. Satake, J. Ohwi, S. Kita- mura, S. Watari and T. Tominari, Eds., Wild Flowers of Japan Vol. I, Heibonsha, Tokyo, 1982, pp. 127-139.
[3] J. Murata, “Should Arisaema serratum Group Be Classified into One or 30 Species? With Reference to the Geographic Structure of Aucuba japonica Widely Distributed in Japan,” Seibutu Kagaku, Vol. 55, No. 2, 2004, pp. 87-94.
[4] J. Murata, “Diversity in the Arisaema serratum Group,” Acta Phytotaxonomica et Geobotanica, Vol. 46, No. 2, 1995, pp. 185-208.
[5] T. Kobayashi, J. Murata and K. Watanabe, “Two New Putative Natural Hybrids in Japanese Arisaema (Araceae),” Acta Phytotaxonomica et Geobotanica, Vol. 56, No. 1, 2005, pp. 105-110.
[6] S. Kobayashi, “Araceae,” In: Kochi Prefecture and Ma- kino Foundation of Kochi Prefecture, Eds., Flora of Ko- chi, Koubun Printing Office, Kochi, 2009, pp. 604-607.
[7] H. Hayakawa, H. Hamachi, Y. Muramatsu, A. Hirata, Y. Minamiya, K. Matsuyama, K. Ito, J. Yokoyama and T. Fukuda, “Interspesific Hybridization between Arisaema sikokianum and A. tosaense (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons,” Acta Phytotaxonomica et Geobotanica, Vol. 61, No. 2, 2010, pp. 57-63.
[8] G. Murata, “Taxonomical Note 7,” Acta Phytotaxonomica et Geobotanica, Vol. 19, No. 2-3, 1962, pp. 67-72.
[9] J. Murata and M. Iijima, “New or Noteworthy Chromo- some Records in Arisaema,” Journal of Japanese Botany, Vol. 58, No. 9, 1983, pp. 270-280.
[10] T. J. White, T. Bruns, S. Lee and J. Taylor, “Amplifica- tion and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics,” In: M. Innis, D. Gelfand, J. Sninsky and T. J. White, Eds., PCR Protocols: A Guide to Methods and Application, Academic Press, San Diego, 1990, pp. 315-322.
[11] P. Taberlet, L. Gielly, G. Pautou and J. Bouvet, “Univer- sal Primers for Amplification of Three Non-Coding Regions of Chloroplast DNA,” Plant Molecular Biology, Vol. 17, No. 15, 1991, pp. 1105-1109. doi:10.1007/BF00037152
[12] J. Murata, “Enumeration of Arisaema in Japan” In: J. Murata, Ed., The Picture Book of Plant Systematics in Color Arisaema in Japan, Hokurikukan, Tokyo, 2011. pp. 71-216.
[13] S. Kakishima, T. Tohma and J. Murata, “Diversity and Crossing in Arisaema serratum Group in Japanese Archipelago,” Annual meeting of Ecological Society of Japan, Vol. 56, 2009, p. 386.

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