Structural rearrangements of polymeric insulin-loaded nanoparticles interacting with surface-supported model lipid membranes

Rickard Frost, Christian Grandfils, Bernardino Cerda, Bengt Kasemo, Sofia Svedhem
DOI: 10.4236/jbnb.2011.22023                

Abstract

The design and screening of nanoparticles for therapeutic applications (nanodrugs) belong to an emerging research area, where surface based analytical techniques are promising tools. This study reports on the interaction of electrostatically assembled nanoparticles, developed for non-invasive administration of human insulin, with cell membrane mimics. Interactions between the nanoparticles and differently charged surface-supported model membranes were studied in real-time with the quartz crystal microbalance with dissipation monitoring (QCM-D) technique, in some experiments combined with optical reflectometry. Based on the experimental observations, we conclude that structural rearrangements of the nanoparticles occur upon adsorption to negatively charged lipid membranes. The degree of nanoparticle deformation will have important implications for the induced release of the protein drug load. The presented results provide an example of how a surface-based experimental platform can be used for evaluation of nanosized drug carriers.

Keywords

nanoparticle, human insulin, drug delivery, supported lipid bilayer, QCM-D, reflectometry, DLS, zeta potential

Conflicts of Interest

The authors declare no conflicts of interest.

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