Functional Analysis for Rolling Leaf of Somaclonal Mutants in Rice (Oryza sativa L.)
Young-Hie Park, Hyun-Suk Lee, Gi-Hwan Yi, Jae-Keun Sohn, Kyung-Min Kim
DOI: 10.4236/ajps.2011.21008   PDF    HTML     6,107 Downloads   12,236 Views   Citations


This study was carried out to facilitate the functional analysis of rice genes. Some 297 insertion plants (1.7%) of the entire lines with the endogenous retrotransposon Tos17 were produced. Phenotypes of these plants in the S2 genera-tion were observed in the field according to different leaf types. Rolling leaf mutants showed thinner sclerenchyma-tous cells, defective arrangement of vascular bundles, and well-formed bulliform cells as compared to the parental cultivar. Two new copies of Tos17 were detected in the rolling leaf type. In the new leaf type, the copy number and activation of Tos12, 15 did not appear as ‘Ilpum’. Flanking sequence tag (FST) analysis of Tos17 in the rolling leaf mutant indicated that new copies of Tos17 were transposed on chromosomes 11 and 12. Annotated homologues of the tagging genes on chromosome 11 were arabinoxylan rabinofuranohydrolase isoenzyme AXAH-I and II. The tagging gene in chromosome 12 was highly correlated with 6 kinds of genes including a transcript regulated factor and a rough sheath 2-like protein. This rolling leaf and flanking sequence data will stimulate the functional analysis of rice genes.

Share and Cite:

Y. Park, H. Lee, G. Yi, J. Sohn and K. Kim, "Functional Analysis for Rolling Leaf of Somaclonal Mutants in Rice (Oryza sativa L.)," American Journal of Plant Sciences, Vol. 2 No. 1, 2011, pp. 56-62. doi: 10.4236/ajps.2011.21008.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] K. Arjun, E. Guiderdoni, G. An, Y. C. Hsing, C. D. Han, M. C. Lee, S. M. Yu, N. Upadhyaya, S. Ramachandran and Q. Zhang, “Mutant Resources in Rice for Functional Genomics of the Grasses,” Plant physiology, Vol. 149, No. 1, 2009, pp. 165-170. doi:10.1104/pp.108.128918
[2] H. G. Chin, M. S. Choe, S. H. Lee, S. H. Park, S. H. Park, J. C. Koo, N. Y. Kim, J. J. Lee, B. G. Oh, G. Yi, S. C. Kim, H. C. Choi, M. J. Cho, C. D. Han, “Molecular Analysis of Rice Plants Harboring an Ac/Ds Transposable Elementmediated Gene Trapping System,” The Plant Journal, Vol. 19, No. 5, 1999, pp. 615-623.
[3] L. Gao, E. M. McCarthy, E. W. Ganko and J. F. McDonald, “Evolutionary History of Oryza sativa LTR Retro- Transposons: A Preliminary Survey of the Rice Genome Sequences,” BMC Genomics, Vol. 5, No. 1, 2004, pp. 1-18. doi:10.1186/1471-2164-5-18
[4] G. Gavazzi, C. Tonelli, G. Todesco, E. arreghini, F. Raf- faldi, F. Vecchio, G. Barbuzzi, M. Biasini and F. Sala, “Somaclonal Variation Versus Chemically Induced Muta- Genesis in Tomato(Lycopersicon esculentum L.),” Theo- retical and Appled Genetics, Vol. 74, No. 6, 1987, pp. 733-738.
[5] Y. M. Govaerts, S. Jacquemoud, M. M. Verstraete and S. L. Ustin, “Three-10 Dimensional Radiation Transfer Modeling in a Dicotyledon Leaf,” Applied Optics, Vol. 35, No. 33, 1996, pp. 6585-6598. doi:10.1364/AO.35.006585
[6] H. Hirochika, A. Fukuchi and F. Kikuchi, “Retrotranspo- son Families in Rice,” Molecular and General Genetics, Vol. 233, No. 1-2, 1992, pp. 209-216. doi:10.1007/BF00587581
[7] H. Hirochika, H. Otsuki, M. Yoshikawa, Y. Otsuki, K. Sugimoto and S. Takeda, “Autonomous Transposition of the Tobacco Retrotransposon Tto 1 in rice,” The Plant Cell, Vol. 8, No. 4, 1996a, pp. 725-734.
[8] H. Hirochika, K. Sugimoto, Y. Otski, H. Tsugawa and M .Kanda, “Retrotransposones of Rice Involved in Muta- tions Induced by Tissue Culture,” Proceedings of National Acadenny of Sciences of the United States of America, Vol. 93, No. 15, 1996b, pp. 7783-7788. doi:10.1073/pnas.93.15.7783
[9] B. O. Juliano, C. M. Perez, A. B. Blakeney, T. Castillo, N. Ongseree, B. Laignelet, E. T. Lapis, V. V. S. Murty, C. M. Paule and B. D. Webb, “International Cooperative Testing on the Amylose Content of Milled Rice,” Starch, Vol. 33, No. 5, 1981, pp. 157-162.
[10] Y. U. Jun, S. Hu, J. Wang, S. Li, K. S. G. Wong, B. Liu, Y. Deng, L. Dai, Y. Zhou, X. Zhang, M. Cao, J. Liu, J. Sun, J. Tang, Y. Chen, X. Huang, W. Lin, C. Ye, W. Tong, L. Cong, J. Geng, Y. Han, L. Li, W. Li, G. Hu, X. Huang, W. Wi, J. Li, Z. Liu, L. Li, J. Liu, Q . Qi, J. Liu, L. Li, X .Wang, H. Lu, T. Wu, M. Zhu, P. Ni, W. Dong, X. Ren, X. Feng, P. Cui, X. Li, H. Wang, X. Xu, W. Zhai, Z. Xu, J. Zhang, S .He, J. Zhang, J. Xu, K. Zhang, X. Zheng, J. Dong, W. Zeng, L. Tao, X. Chen, J. He, D. Liu, W. Tian,
[11] M. Kawai, S. Kidou, A. Kato, H. Uchimiya, “Molecular Characterization of cDNA Encoding for Adenylate Kinase of Rice (Oryza sativa L.),” The Plant Journal, Vol. 2, No. 6, 1992, pp. 845-854.
[12] G. S. Khush and T. Kinoshita, “Rice Karyotype, Marker Genes, and Linkage Group,” Rice Biotechnology, CAB International, Wallingford, 1991, pp. 83-107.
[13] T. Kinoshita, “Gene Analysis and Linkage Map,” Biology of Rice, JSSP/Elsevier, Tokyo, 1984 pp. 187-274.
[14] P. J. Larkin, P. M. Bank, R. Bhati, R. I. S. Brettelli, P. A. Davies, S. A. Ryan, W. R. Scowcroft, L. H. Spindle and G. T. Tanner, “From Somatic Variant to Variant Plants: Mechanisms and Applications,” Genomes, Vol. 31, No. 2, 1989, pp. 705-711.
[15] P. J. Larkin and W. R. Scowcroft, “Somaclonal Variation—A Novel Source of Variability from Cell Cultures for Plant Improvement,” Theoretical and Applied Genetics, Vol. 60, No. 4, 1981, pp. 197-214. doi:10.1007/BF02342540
[16] S. G. Li, Y. Q. Ma, P. He, H. Y. Li, Y. Chen, K. D. Zhou and L. H. Zhu, “Genetics Analysis and Mapping the Flag Leaf Roll in Rice (Oryza. sativa. L.),” Journal Sichuan Ag- riculture University, Vol. 16, 1998, pp. 391-393.
[17] Z. Luo, Z. Yang, B. Zhong, Y. Li, R. Xie, F. Zhao, Y. Ling and G. He, “Genetic Analysis and Fine Mapping of a Dynamic Rolled Leaf Gene, RL 10(t), in Rice (Oryza. sativa. L.),” Genome, Vol. 50, No. 9, 2007, pp. 811-817. doi:10.1139/G07-064
[18] A. Miyao, Y. Iwasaki, H. Kitano, J. I. Itoh, M. Maekawa, K. Murata, O. Yatou, Y. Nagato and H. Hirochika, “A Large-Scale Collection of Phenotypic Data Describing an Insertional Mutant Population to Facilitate Functional Analysis of Rice Genes,” Plant Molecular Biology , Vol. 63, No. 5, 2007, pp. 625-635. doi:10.1007/s11103-006-9118-7
[19] T. Murashige and F. Skoog, “A revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Physioogial Plantarum, Vol. 15, No. 3, 16 1962, pp. 473-497.
[20] K. M. Nori, K. Miyoshi, K. I. Nonomura, Y. Yamazaki and Y. Ito, “Rice Mutants and Genes Related to Organ Development, Morphogenesis and Physiological Traits,” Plant Cell Physiology, Vol. 46, No. 1, 2005, pp. 48-62. doi:10.1093/pcp/pci506
[21] Y. j. Shao, Z. X. Chen, Y. F. Zhang, E. H. Chen, D. C. Qi, J. Miao and X. B. Pan, “One Major QTL Mapping and Physical Map Construction for Rolled Leaf in Rice,” Acta Genetica Sinica (in Chinese), Vol. 32, No. 5, 2005a, pp. 501-506.
[22] Y. J. Shao, C. H. Pan, Z. X. Chen, S. M. Zuo, Y. F. Zhang and X. B. Pan, “Fine Mapping of an Incomplete Recessive Gene for Leaf Rolling in Rice(Oryza. sativa. L.),” Chiness Science Bulletin (in Chinese), Vol. 50, No. 21, 2005b, pp. 2466-2472.
[23] Z. Y. Shi, J. Wang, X. S. Wan, G. Z. Shen, X. Q. Wang and J. l. Zhang, “Over-Expression of Rice OsAGO 7 Gene Induces Upward Curling of the Leaf Blade that Enhanced Erect-Leaf Habit,” Planta, Vol. 226, No. 1, 2007, pp. 99-108. doi:10.1007/s00425-006-0472-0
[24] K. Sugimoto, Y. Otsuki, S. Saji and H. Hirochika, “Transposition of the Maize Ds Element from a Viral Vector to the Rice Genome,” The Plant Journal, Vol. 5, No. 6, 1994, pp. 863-871. doi:10.1046/j.1365-313X.1994.5060863.x
[25] B. Winnepenninckx, T. Backeljau and R. De Wachter, “Extraction of high molecular weight DNA from mollusks,” Trends in Genetics, Vol. 9, No. 12, 1993, pp. 407. doi:10.1016/0168-9525(93)90102-N
[26] Q. J. Xie, M. C. Ruch and J. H. Oard, “Homozygous Variation in Rice Somaclones: Non Random Variation In- stead of Mitotic Recombination,” Crop Science, Vol. 35, No. 4, 1995, pp. 954-957. doi:10.2135/cropsci1995.0011183X003500040002x
[27] C. J. Yan, S. Yan, Z. Q. Zhang, G. H. Liang, J. F. Lu and M. H. Gu, “Genetic Analysis and Gene Fine Mapping for a Rice Novel Mutant rl 9(t) with Rolling Leaf Character,” Chi- ness Science Bulletin (in Chinese), Vol. 51, No. 1, 2006, pp. 63-69.
[28] G. H. Zhang, Q. Xu, X. D. Zhu, Q. Qian and H. W. Xue, “Shallot-Like is a KANDI Transcription Factor that Modulates Rice Leaf Rolling by Regulating Leaf Abaxial Cell Development,” The Plant Cell Preview, Vol. 21, No. 3, 2009, pp. 719-735. doi:10.1105/tpc.108.061457

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.