The comparison of the efficacy of swine FMD vaccine emulsified with oil adjuvant of ISA 201 VG or ISA 206 VG


The Seppic Company developed a new adjuvant Montanide ISA 201 VG, the upgraded version of Montanide ISA 206 VG, which keep the advantage and added some chemical components on the basis of ISA 206 to improve the cellular responses. The aim of the study is to compare the efficacy of swine FMD (foot-and-mouth) vaccine emulsified with oil adjuvant of ISA 201 or ISA 206 respectively. The pigs were vaccinated with FMD vaccine emulsified with inactive FMD type O antigen and adjuvant ISA 201 or ISA 206 respectively, according to 2.0 ml (1/1 dose), 0.67 ml (1/3 dose), 0.22 ml (1/9 dose) to calculate their PD50. The sera were collected from the vaccination of the day 0, 3, 7, 14, 21, 28 and the ELISA FMD type O antibody were detected. Furthermore, the PD50 were calculated after the pigs were challenged with virulent FMDV type O on 28 days post vaccination. The ELISA antibody titers of 201vaccine were significantly higher than that of 206 (except the third time). The fifty percent of protection dose (PD50) of 201 vaccine (PD50 = 15.59) was higher than that of 206 vaccine (PD50 = 10.05). The above data showed that the efficacy of the FMD vaccine emulsified with ISA 201 was better than which with ISA 206.

Share and Cite:

Li, D. , Zhou, C. , She, D. , Li, P. , Sun, P. , Bai, X. , Chen, Y. , Xie, B. and Liu, Z. (2013) The comparison of the efficacy of swine FMD vaccine emulsified with oil adjuvant of ISA 201 VG or ISA 206 VG. Journal of Biosciences and Medicines, 1, 22-25. doi: 10.4236/jbm.2013.13005.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Reuckert, R.R. (1996) Picornaviridae: the viruses and their replication. In: Fields, B.N., Knipe, D.M. and Howley, P.M. Eds., Fields Virology, Lippincott-Raven, Philadelphia, 609-654.
[2] Doel, T.R. (2003) FMD vaccines. Virus Research, 91, 81- 99.
[3] Ward, M.P., Laffan, S.W. and Highfield, L.D. (2007) The potential role of wild and feral animals as reservoirs of foot-and-mouth disease. Preventive Veterinary Medicine, 80, 9-23.
[4] Doel, T.R., Williams, L. and Barnet, P. (1994) Emergency vaccination against foot-and-mouth disease: Rate of development of immunity and its implications for the carrier state. Vaccine, 12, 592-600.
[5] Barnett, P.V., Pullen, L., Williams, L. and Doel T.R. (1996) International bank for foot-and-mouth disease: Assessment of Montanide ISA 25 and ISA 206, two commercially available oil adjuvants. Vaccine, 14, 1187- 1198.
[6] Hunter, P. (1996) The performance of southern African territories serotypes of foot and mouth disease antigen in oil-adjuvanted vaccines. Revue Scientifique et Technique, 15, 913-922.
[7] Cox, S.J., Barnett, P.V., Dani, P. and Salt J.S. (1999) Emergency vaccination of sheep against foot-and-mouth disease: Protection against disease and reduction in contact transmission. Vaccine, 17, 1858-1868.
[8] Doel, T.R. and Pullen, L. (1990) International bank for foot-and-mouth disease vaccine: stability studies with virus concentrates and vaccines prepared from them. Vaccine, 8, 473-478.
[9] Barnett, P.V. and Carabin, H. (2002) A review of emergency foot-and-mouth disease (FMD) vaccines. Vaccine, 20, 1505-1514.
[10] Sonia, A.M. (2007) Studies on preparation improved FMD virus oil adjuvant vaccine. VMVSc Thesis (Virology), University of Cairo, Cairo.
[11] Fatthia, A.M. (2003) Vaccination of goats with FMD vaccines. VMVSc Thesis (Infectious Disease), University of Alexandria, Alexandria.
[12] Mohamed, A.A. (1998) Study on new double oil adjuvant for FMD virus vaccine. VMVSc Thesis (Virology), University of Cairo, Cairo.
[13] Wafaa, E.H. (1999) Epizootiological and immunological Studies on Foot and Mouth Disease. Ph.D. Thesis (Infectious Disease), University of Cairo, Cairo.
[14] Daoud, A.M., Ali, S.M. and Yousef, M.R. (2002) Comparative study between different new oil adjuvants used for production of FMD vaccine in sheep. 4th International Science Conference, 667-673.
[15] Patil, P.K., Bayry, J., Ramakrishna, C., Hugar, B., Misra, L.D. and Prabhudas, K. (2002) Immune responses of sheep to quadrivalent double emulsion foot-and-mouth disease vaccines: Rate of development of immunity and variations among other ruminants. Journal of Clinical Microbiology, 40, 4367-4371.
[18] Li, D., Lu, Z.J., Xie, B.X., Sun, P., Chen, Y.L., Fu, Y.F. and Liu, Z.X. (2010) Alternative way to test the efficacy of swine FMD vaccines: Regulation of live virus challenge dose. Virology Journal, 7, 215.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.