Preparation of Water Soluble Yeast Glucan by Four Kinds of Solubilizing Processes

Liping Du, Xuekuang Zhang, Chao Wang, Dongguang Xiao

Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology Tianjin University of Science and Technology, Tianjin University of Science and Technology, Tianjin 300457, P. R. China.

DOI: 10.4236/eng.2012.410B048                

Abstract

(1→3)-β-D-glucan from the inner cell wall of Saccharomyces cerevisiae is considered a member of a class of drugs known as biological response modifiers (BRM). However the glucan was an insoluble polysaccharide, which could be the major barrier to the utilization of glucan. In this case, the insoluble glucan was convent into a soluble form by four kind of solubilizing processes. The yield, solubility, chemistry structure and immunoprophylaxis efficacy of the soluble products were compared. Our date suggest that urea has a significant effect on yield, and DMSO has a significant effect on solubility. FT-IR spectra, 13C NMR spectra and helix-coil transition analysis demonstrate that the chemistry structure of native and solubilizing glucans have no significant difference. They still have the triple helical structure. The solubility and immunoprophylaxis efficacy assay indicate that the introduction of phosphate group not only enhanced the solubility of glucan, but also improved the survival rate of mice challenged with E. coli.

Keywords

Glucan; Solubility; FT-IR Spectra; 13C NMR Spectra; Immunoprophylaxis Efficacy

Conflicts of Interest

The authors declare no conflicts of interest.

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