Susceptibility of Culex quinquefasciatus (Diptera: Culicidae) to malathion in Sargodha district, Pakistan


The present study was designed to evaluate the susceptibility of Culex quinquefasciatuns against malathion in Sargodha district of Pakistan. The activities of detoxifying enzymes i.e., β-esterases, GSTs and monooxygenases were also estimated. Our results revealed 100 percent mortality in insecticide treated groups after 24 hours of exposure. There was no difference in the activities of insecticide detoxifying enzymes between control and treated groups. We concluded from our work that malathion is still effective against C. quinquefasciatuns in the area for control of mosquito population.

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Tahir, H. , Hussain, K. , Khan, A. , Naseem, S. , Malik, H. , Butt, A. and Yaqoob, R. (2013) Susceptibility of Culex quinquefasciatus (Diptera: Culicidae) to malathion in Sargodha district, Pakistan. Open Journal of Animal Sciences, 3, 1-4. doi: 10.4236/ojas.2013.34A1001.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] World Health Organisation (2011) Lymphatic filariasis.
[2] Abdel-Hameed, A.A., Dura, W.T. and Alkhalife, I.S. (2004) An inguinal mass with local vascular lesions induced by a lymphatic filaria. Saudi Medical Journal, 25, 1106-1108.
[3] McCarroll, L. and Hemingway, J. (2002) Can insecticide resistance status affects parasite transmission in mosquitoes? Insect Biochemistry and Molecular Biology, 32, 1345-1351.
[4] Chareonviriyaphap, T., Sungvornyothin, S., Ratanatham, S.S. and Prabaripai, A. (2001) Insecticides induced behavioural responses of Anopheles minimus, a malarial vector in Thailand. Journal of American Mosquito Control Association, 17, 13-22.
[5] Lee, H.L. (2005) Vector of filariasis in Malaysia—A review. In: Kimura, E., Rim, H.J., Dejian, S. and Weerasooriya, M.V., Eds., Asian Parasitology Vol. 3 Filariasis in Asia and Western Pacific Islands. FAP Journal Ltd., Japan.
[6] Wondji, C.S., Priyanka De Silva, W.A., Hemingway, J., Ranson, H. and Parakrama Karunaratne, S.H. (2008) Characterization of knockdown resistance in DDT-and pyrethroid-resistant Culex quinquefasciatus populations from Sri Lanka. Tropical Medicines and International Health, 13, 548-555.
[7] Hemingway, J., Hawkes, N.J., McCarroll, L. and Ranson, H. (2004) The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology, 34, 653-665.
[8] Hemingway, J. and Ranson, H. (2000) Insecticide resistance in insect vectors of human disease. Annual Review of Entomology, 45, 371-391.
[9] Enayati, A.A., Ranson, H. and Hemingway, J. (2005) Insect glutathione transferases and insecticide resistance. Insect Molecular Biology, 14, 3-8.
[10] Li, X., Schuler, M.A. and Berenbanm, R. (2007) Molecular mechanism of metabolic resistance to synthetic and natural xenobioatics. Annual Review of Entomology, 52, 231-253.
[11] Baker, J.E., Fabrick, J.A. and Zhu, K.Y. (1998) Characterization of esterases in malathion resistant and susceptible strains of a pteromalid parasitoid, Anisopteromalus calandra. Insect Biochemistry and Molecular Biology, 28, 1039-1050.
[12] Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974) Glutathione S-transferase, the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249, 7130-7139.
[13] Vulule, J.M., Beach, R.F., Atieli, F.K., McAllister, J.C., Brogdon, W.G., Roberts, J.M., Mwangi, R.W. and Hawley, W.A. (1999) Elevated oxidase and esterases levels associated with permethrin tolerance in Anopheles gambie from Kenyan villages using permethrin impregnated nets. Medical and Veterinary Entomology, 13, 239-244.
[14] Roth, D., Henry, B., Mak, S., Fraser, M., Taylor, M., Li, M., Cooper, K., Furnell, A., Wong, Q., Morshed, M. and Members of the British Columbia West Nile Virus Surveillance Team (2010) West Nile virus range expansion into British Columbia. Emerging Infectious Diseases, 16, 1251-1258.
[15] Duran, M. and Stevenson, H. R. (1983) Insecticide resistance in adult Culex quinquefasciatus mosquitoes from Olongapo City, Philippines. Southeast Asian Journal of Tropical Medicines and. Public Health, 14, 403-406.
[16] Hamdan, H., Sofian-Azirun, M., Ahmad, N.W. and Lee, L.H. (2005) Insecticide resistance development in Culex quinquefasciatus (Say), Aedes aegypti (L.) and Aedes albopictus (Skuse) larvae against malathion, permethrin and temephos. Tropical Biomedicines, 22, 45-52.
[17] Kumar, K., Sharma, A.K., Kumar, S., Patel, S., Sarkar, M. and Chauhan, L.S. (2011) Multiple insecticide resistance/ susceptibility status of Culex quinquefasciatus, principal vector of bancroftian filariasis from filarial endemic areas of northern India. Asian Pacific Journal of Tropical Medicine, 4, 426-429.
[18] Radwan, E.M.M. (2012) Malathion resistance and acetylcholinesterase enzyme changes in field population of the peach fruit fly, Bactrocera zonata (Saunders). American Journal of Sciences, 8, 1042-1053.
[19] Bong, L.J. and Zairi, J. (2010) Temporal fluctuations of insecticides resistance in Musca domestica Linn (Diptera: Muscidae) in Malaysia. Tropical Biomedicine, 327, 317-325.
[20] Bossard, R., Hinkle, N.C. and Rust, M.K. (1998) Review of insecticide resistance in cat flies (Siphonaptera: Puhcidae). Journal of Medical Entomology, 5, 415-422.

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