Development of a SCAR Marker for Discrimination of a Thai Jasmine Rice (Oryza sativa L. cv. KDML105) Mutant, BKOS6, and Associated with Purple Color Trait in Thai Jasmine Rice-Related Varieties

Nuananong Semsang, Rattaporn Chundet, Boonrak Phanchisri

Department of Biology, Faculty of Science and Technology, Chiang Mai Rajabhat University, Chiang Mai, Thailand;.
Department of Biology, Faculty of Science, Maejo University, Chiang Mai, Thailand;.
Science and Technology Research Institute, Chiang Mai University, Chiang Mai, Thailand..

DOI: 10.4236/ajps.2013.49218                

Abstract

The potential of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The improved rice variety, BKOS6, exhibited many remarkable characteristics which fitted the multiple cropping system characteristics of progressive agriculture including photoperiod insensitivity, early flowering, short in stature, and purple pigment accumulation in pericarp. The BKOS6 rice grain extract has already been proved that it exhibited higher antioxidant properties than the KDML 105 and other tested rice grain extracts. In this study, the BKOS6 specific SCAR marker was developed by HAT-RAPD analysis of rice genomic DNA. The marker was successfully used to identify BKOS6 variety and its hybrid varieties containing purple pigment accumulation in plant tissues. Moreover, it was found that this marker could be used to detect other purple pigmented rice varieties that genetically related to Thai jasmine rice. Recently, a wide variety of anthocyanin-based foods are believed to provide significant potential health benefits, and become more attractive. KDML 105 is also a Thai premier fragrant rice variety which is one of the main varieties of country’s rice export. Thus this molecular marker could be useful for commercial and breeding purposes of BKOS6 mutant and other developed varieties from KDML 105 which contain anthocyanin accumulation.

Keywords

SCAR Marker; KDML 105 Mutant; Low-Energy Ion Beam; Anthocyanin Accumulation; Purple Pigmented Rice

Conflicts of Interest

The authors declare no conflicts of interest.

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