Endophytic Mycobiota Characterization of the Amazonian Mistletoe Cladocolea micrantha Hosted in Cashew Tree

Anderson C. Guimarães, Antonio C. Siani, José L. Bezerra, Antonia Q. Lima de Souza, Maria Inez M. Sarquis

Departamento de Ciências Agron?micas e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, Brazil.
Departamento de Produtos Naturais, Farmanguinhos, Fiocruz, Rio de Janeiro, Brazil.
Instituto de Ciências Exatas e Tecnologia de Itacoatiara, Universidade Federal do Amazonas, Itacoatiara, Brazil.
Laboratório de Genética, Escola Superior de Ciências da Saúde, Universidade Estadual do Amazonas, Manaus, Brazil.
Laboratório de Taxonomia Bioquímica e Bioprospec??o de Fungos, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.

DOI: 10.4236/ajps.2013.44113                

Abstract

Endophytic fungi were identified from different parts of the medicinal parasitic mistletoe Cladocolea micrantha and from its host Anacardium occidentale, suggesting a strict host-parasite relationship. Eight fungal endophytes were isolated and morphologically characterized. The ascomycete Guignardia mangiferae and strains of Mycelia sterilia were prevalent in the isolations. The unequivocal identification of Guignardia mangiferae at a probabilistic degree close to 100% was carried out by DNA extraction followed by PCR analyses of the ITS-1 and ITS-2 regions and comparison of the genetic sequence with the NCBI database.

Keywords

Cladocolea micrantha; Endophytic mycobiota; Loranthaceae; Guignardia mangiferae ; Anacardium occidentale

Conflicts of Interest

The authors declare no conflicts of interest.

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