Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis


Some Angelica species are used for medicinal purposes. In particular, the roots of Angelica acutiloba var. acutiloba and A. acutiloba var. sugiyamae, known as Toki and Hokkai Toki, respectively, are used as important medicinal materials in traditional Japanese medicine. However, since these varieties have recently outcrossed with each other, it is difficult to determine whether the Japanese Angelica Root material used as a crude drug is the pure variety. In this study, we developed an efficient method to authenticate A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae from each other and from other Angelica species/varieties. The random amplified polymorphic DNA (RAPD) method efficiently discriminated each Angelica variety. A. acutiloba var. sugiyamae was identified via a characteristic fragment amplified by the decamer primer OPD-15. This fragment showed polymorphisms among Angelica species/varieties. The unique fragment derived from A. acutiloba var. sugiyamae was also found in one strain of A. acutiloba var. acutiloba, implying that this strain arose from outcrossing between A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae. This RAPD marker technique will be useful for practical and accurate authentication among A. acutiloba var. acutiloba, A. acutiloba var. sugiyamae, and their adulterants.

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K. Matsubara, S. Shindo, H. Watanabe and F. Ikegami, "Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis," American Journal of Plant Sciences, Vol. 4 No. 2, 2013, pp. 269-273. doi: 10.4236/ajps.2013.42035.

Conflicts of Interest

The authors declare no conflicts of interest.


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