Comparison of Photosynthetic Pigment Contents of the Resurrection Plants Ramonda serbica and Ramonda nathaliae of Some Different Populations from Kosovo, Albania and Macedonia


The resurrection plants Ramonda serbica and Ramonda nathaliae are the physiological model plant and endemic species from Balkan Peninsula. Study was carried out to assess the impact of different populations on photosynthetic pigment contents and their effect on physiological activity of these species. The experiment was conducted with leaves of R. serbica collected from nine populations in Kosovo, three in Albania and two in Macedonia, while for R. nathaliae four populations in Macedonia. For this purpose plants after collection from their natural populations were kept for 24 hours at constant temperature and humidity and in natural photoperiod. According to our data the pigment contents (Total chl + Carot) of R. serbica, measured as mg per gram dry weight (DW), were higher (7.06 mg·g-1 DW) in Radaci populations (Kosovo) and lower (4.63 mg·g-1 DW) in Jukniu Mountain-Kruja (Albania). On the other hand, the higher (5.28 mg·g-1 DW) of pigment contents (Total chl + Carot) of R. nathaliae were observed in Vorca populations and the lower (4.24 mg·g-1 DW) in Kaparll?k populations. Ratio chlorophyll a/b of R. serbica in Kruja Castle populations from Albania was the higher (3.68) comparing with Zhlebi populations from Kosovo (1.68). Similarly in case of R. nathaliae the higher (3.36) value was in Kaparll?k populations and the lower (3.12) in Matka populations. The data obtained were further analyzed using one-way ANOVA and a significant change was recorded in the different populations. These studies clearly indicate that the Ramonda plants from different ecological habitats there have been changes of photosynthetic pigment contents.

Share and Cite:

B. Gashi, K. Abdullai and E. Kongjika, "Comparison of Photosynthetic Pigment Contents of the Resurrection Plants Ramonda serbica and Ramonda nathaliae of Some Different Populations from Kosovo, Albania and Macedonia," American Journal of Plant Sciences, Vol. 3 No. 11, 2012, pp. 1588-1593. doi: 10.4236/ajps.2012.311192.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Z. Tuba, M. C. F. Proctor and Z. Csintalan, “Ecophysiological Responses of Homoichlorophyllous and Poikilochlorophyllous Desiccation-Tolerant Plant Xerophyta scabrica at Present Day CO2 Concentration,” Journal of Plant Physiology and Biochemistry, Vol. 35, 1998, pp. 381-386.
[2] Y. K. Markovska, T. D. Tsonev, G. P. Kimenov and A. A. Tutekova, “Physiological Changes in Higher Poikilohydric Plants—Haberlea rhodopensis Friv. and Ramonda Serbica Panc. during Drought and Rewatering at Different Light Regimes,” Journal of Plant Physiology, Vol. 144, No. 1, 1994, pp. 100-108. doi:10.1016/S0176-1617(11)81000-X
[3] A. Augusti, A. Scartazza, F. Navari-Izzo, C. L. M. Sgherri, B. Stevanovic and E. Brugnoli, “Photosystem II Photochemical Efficiency, Zeaxanthin and Antioxidant Contents in the Poikilohydric Ramonda serbica during Dehydration and Rehydration,” Photosynthesis Research, Vol. 67, No. 1-2, 2001, pp. 79-88. doi:10.1023/A:1010692632408
[4] M. F. Quartacci, O. Glisic, B. Stevanovic and F. Navari-Izzo, “Plasma Membrane Lipids in the Resurrection Plant Ramonda serbica Following Dehydration and Rehydration,” Journal of Experimental Botany, Vol. 53, No. 378, 2002, pp. 2159-2166. doi:10.1093/jxb/erf076
[5] C. Sgherri, B. Stevanovic and F. Navari-Izzo, “Role of Phenolics in the Antioxidative Status of the Resurrection Plant Ramonda serbica during Dehydration and Rehydration,” Physiologia Plantarum, Vol. 122, No. 4, 2004, pp. 478-485. doi:10.1111/j.1399-3054.2004.00428.x
[6] Z. Jovanovic, T. Rakic, B. Stevanovic and S. Radovic, “Characterization of Oxidative and Antioxidative Events during Dehydration and Rehydration of Resurrection Plant Ramonda nathaliae,” Journal of Plant Growth Regulator, Vol. 64, No. 3, 2011, pp. 231-240. doi:10.1007/s10725-011-9563-4
[7] E. Degl’Innocenti, L. Guidi, B. Stevanovic and F. Navari, “CO2 Fixation and Chlorophyll a Fluorescence in Leaves of Ramonda serbica during a Dehydration-Rehydration Cycle,” Journal of Plant Physiology, Vol. 165, No. 7, 2008, pp. 723-733. doi:10.1016/j.jplph.2007.06.009
[8] S. Yakovlev-Siljak, V. Stevanovic, M. Tomasevic, C. S. Brown and B. Stevanovic, “Genome Size Variation and Polyploidy in the Resurrection Plant Genus Ramonda: Cytogeography of Living Fossils,” Environmental and Experimental Botany, Vol. 62, No. 2, 2008, pp. 101-112. doi:10.1016/j.envexpbot.2007.07.017
[9] T. Zivkovic, M. F. Quartacci, B. Stevanovic, F. Marinone and F. Navari-Izzo, “Low Molecular Weight Substances in the Poikilohydric Plant Ramonda serbica during Dehydrationand Rehydration,” Plant Science, Vol. 168, No. 1, 2005, pp. 105-111. doi: 10.1016/j.plantsci.2004.07.018
[10] B. Gashi, K. Abdullai, V. Mata and E. Kongjika, “Effect of Gibberellic Acid and Potassium Nitrate on Seed Germination of the Resurrection Plants Ramonda serbica and Ramonda nathaliae,” African Journal of Biotechnology, Vol. 11, No. 20, 2012, pp. 4537-4542.
[11] E. Kongjika, Zh. Zekaj, E. ?aushi and I. Stamo, “Plant Biotechnology—In Vitro Culture,” Academy of Science, Institute of Biological Researchers, Tirana, 2002, pp. 119-140.
[12] S. Dontcheva, E. Daskalova, G. Yahubyan, I. Denev, I. Minkov and V. Toneva, “Conservation of the Protected Resurrection Species Ramonda serbica Panc.—Habitat Montana District, Bulgaria as in Vitro Plants through a Modified Micropropagation System,” Biotechnology and Biotechnological Equipment, Vol. 23, No. 2, 2009, pp. 369-372.
[13] B. Gashi, K. Abdullai, V. Mata, S. Misimi, M. Osmani and E. Kongjika, “In Vitro Culture—A Tool to Overcome the Poor in Vivo Development of Genus Ramonda Plants,” Bulletin of Natural Science, Special Edition, 2011, pp. 536-543.
[14] H. Lichtenthaler, “Laser-Induced Chlorophyll Fluorescence of Living Plants,” Proceedings of the Remote Sensing Symposium, Band III, ESA Publication Division, Nordwijk, 1986, pp. 1571-1579.
[15] G. A. F. Hendry and A. H. Price, “Stress Indicators: Chlorophylls and Carotenoids,” In: G. A. F. Hendry, Ed., Methods in Comparative Plant Ecology, Chapman & Hall, London, 1993, pp. 148-152. doi:10.1016/j.plantsci.2004.07.018
[16] J. F. C. Gon?alves, R. A. Marenco and G. Vieira, “Concetration of Photosynthetic Pigments and Chlorophyll Flourescence of Mahogany and Tonka Bean under Two Light Environments,” Revista Brasileira de Fisiologia Vegetal, Vol. 13, No. 2, 2001, pp. 149-157. doi:10.1590/S0103-31312001000200004
[17] Y. Tan, J. H. Jiang, H. L. Wu, H. Cui and R. Q. Yu, “Resolution of Kinetic System of Simultaneous Degradation of Chlorophyll a and b by PARAFAC,” Analytica Chimica Acta, Vol. 412, 2000, pp. 195-202. doi:10.1016/S0003-2670(99)00813-2
[18] K. N. Boardman, “Comparative Photosynthesis of Sun and Shade Plants,” Annual Review of Plant Physiology, Vol. 28, 1977, pp. 355-377. doi:10.1146/annurev.pp.28.060177.002035

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.