Anti-diabetic effects of cold and hot extracted virgin coconut oil
Mahadevappa Siddalingaswamy, Arunchand Rayaorth, Farhath Khanum
DOI: 10.4236/jdm.2011.14016   PDF    HTML     8,805 Downloads   18,642 Views   Citations


Virgin coconut oil (VCO) has been shown to possess insulinotropic effects shown in isolated perfused mouse islet with hypolipidemic effects. Hot extracted virgin coconut oil (HEVCO) has been shown to possess better antioxidant properties than cold extracted virgin coconut oil (CEVCO). These properties were exploited to study the anti-diabetic effects of HEVCO and CEVCO in diabetic rats. Four groups 8 rats each, first group served as non-diabetic control remaining groups were made diabetic and force fed with 2ml alcoholic extracts of commercial coconut oil (CCO), CEVCO and HEVCO for 21 days. Blood glucose once in 5 days, body weight gain, food intake once in a week and water intake and urine output daily, were monitored. Animals were sacrificed at the end of 21 days. The results indicated HEVCO reduced blood glucose and lipids viz total cholesterol (TC), triglycerides (TG), High density lipoproteins (HDL), Low and Very Low Density Lipoprotein (LDL+ VLDL) and thiobarbutyric acid reactive substances (TBARS) increased the antioxidant status by elevating activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), glutathione (GSH) concentration and decresed lipid peroxidation in liver than CEVCO. These beneficial effects may be attributed to increased polyphenolic and other antioxidants content present in HEVCO.

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Siddalingaswamy, M. , Rayaorth, A. and Khanum, F. (2011) Anti-diabetic effects of cold and hot extracted virgin coconut oil. Journal of Diabetes Mellitus, 1, 118-123. doi: 10.4236/jdm.2011.14016.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Fife, B. (2005) Coconut cures: Preventing and treating common health problems with coconut. Piccadilly Books, Ltd., London, 184-185.
[2] Ohkawa, H., Ohishi, N. and Yagi, K. (1979) Assay of lipid peroxidation in animal tissue by thiobarbutyric acid reaction. Analytical Biochemistry, 95, 351-358. doi:10.1016/0003-2697(79)90738-3
[3] Girotti, A.W. and Thomas, I.P. (1984) Damage effects of oxygen radicals on resealed erythrocyte ghosts. The Journal of Biological Chemistry, 259, 1744-1752.
[4] Flohe, L. and Otting, F. (1984) Superoxide assay. Methods in Enzymology, 105, 93-104.
[5] Cohen, G., Dembiec, C. and Marens, J. (1970) Measurement of catalase activity in tissue extracts. Analytical Biochemistry, 34, 30-38.
[6] Weiss, C., Maker, H.S. and Lehrer, G.M. (1980) Sensitive fluormetric assays for glutathione peroxidase and reductase. Analytical Biochemistry, 106, 512-516.
[7] Ellman, G.L. (1958) A colorimetric method for low concentration of mercaptons. Archives of Biochemistry and Biophysics, 74, 443-450.
[8] St-Onge, M.P., Ross, R., Parsons, W.D. and Jones, P.J. (2003) Medium chain—Triglycerides increases energy expenses and decreases adiposity in over weight men. Obesity Research, 11, 395-402. doi:10.1038/oby.2003.53
[9] Khor, H.T, Rajendran, R. and Gopalakrishnan, M. (1998) The role of unsaponifiable components in the lypidemic property of olive oil. Malaysian Journal of Nutrition, 4, 73-80.
[10] Han, J., Hamilton, J.A., Kirkland, J.L., Corkey, B.E., Guo, W. (2003) Medium chain oil reduces fat mass and down regulates expression of adipogenic genes in rats. Obesity Research, 11, 734-744.
[11] Balasubramanyan, P., Parit, L. and Venugopal, P.M. (1998) Protective effects of carrot against Lindane induced hypatotoxicity in rats. Phytotherapy Research, 12, 434-436. doi:10.1002/(SICI)1099-1573(199809)
[12] Bureau of Product Standards (BPS) (2004) Philippine national standard. Virgin Coconut Oil (VCO), Philippines.
[13] Lee, S.H., Jeong, T. Young, Parth, Y.B., Kwong, Y.C., Choi, M.S. and Bole, S.H. (1999) Hypocholestremic effect of hesperedin mediated by inhibition of 3 hydroxy 3 methyl glutoryl coenzyme A reductase and acyl coenzyme A cholesterol acyl transferase in rats. Nutrition Research, 19, 1245-1258.
[14] Garfinkel M., Lee, S. and Opara, E.C. (1992) Insulinotropic potency of lauric acid: A metabolic rational for medium chain fatty acids (MCF) in TPN formation. Journal of Surgical Research, 52, 328-333. doi:10.1016/0022-4804(92)90111-C
[15] Saneviratane, K.N., Hapurachch, C.D. and Ekanayake, S. (2009) Comparision of the phenolic dependant antioxidant properties of coconut oil extracted under cold and hot conditions. Food Chemistry, 114, 1444-1449. doi:10.1016/j.foodchem.2008.11.038
[16] Nevin, K.G. and Rajamohan, T. (2004) Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clinical Biochemistry, 37, 830-835.
[17] Nevin, K.G. and Rajamohan, T. (2006) Virgin coconut oil supplemented diet increases the antioxidant status in rats, Food Chemistry, 99, 260-266. doi:10.1016/j.foodchem.2005.06.056
[18] Evans, J.L. (2007) Antioxidants: Do they have a role in the treatment of insulin resistance. Indian Journal of Medical Research, 125, 355-372.
[19] Perry, N.B., Bergess, E.J. and Glennic, V.I. (2001) Echinacca standardization: Analytical methods for phenolic compounds and typical levels in medicinal species. Journal of Agricultural and Food Chemistry, 49, 1702-1706. doi:10.1021/jf001331y
[20] Hetono, T., Kagania, H., Yasuhara, T. and Okuda, T. (1988) Two new flavonoids and other constituents in licorice roots: Their relative astringency and radical scavenging effects. Chemical & Pharmaceutical Bulletin, 36, 2090-2097.

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