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Effect of Soil Physico-Chemical Properties and Plant Type on Bacterial Diversity in Semi-Arid Parts in Central Sudan. Part I: Omdurman North Region

DOI: 10.4236/oalib.1101863    766 Downloads   1,208 Views   Citations

ABSTRACT

Bacterial diversity and total viable counts of bacteria of the different soil samples from two different localities in Omdurman north region—Karary and Khor Omer sub-regions—were carried out. Soil physical and chemical characteristics (pH, EC, SP, solublecations: Na, K, Ca, Mg and anion P, organic carbon, total nitrogen and soil texture) in each studied sub-regions were measured. Qualitative analysis of microorganisms isolated from the studied soil samples reveals a total of more than eight different species of bacteria, of which two are unidentified. The six species are classified under Bacillus genera. In Omdurman north region soil samples, total bacterial counts ranged from 7.5 × 103 cfu·g﹣1 to 1 × 104 with a mean of 4 × 104 cfu·g﹣1. The quantitative data on microbial population recorded in the present study were analysed using two diversity indices. High Shannon-Weiner diversity index value for bacteria was obtained in Khor Omer sub-region (1.71261). Actinomyces spp. and Streptomyces spp. were the most abundant microorganisms identified in the two sub-regions. Total bacterial count in Karary soil was positively correlated with EC (r = 0.3868), clay (r = 0.1412), sand (r = 0.5891) and K (r = 0.0265) and negatively correlated with pH, silt, SP, Na, P, Ca, Mg, N and OC whereas the total bacterial count in Khor Omer soil was positively correlated with EC (r = 0.3973), clay (r = 0.1966), silt (r = 0.2116), Ca (r = 0.6733), Mg (r = 0.586) and OC (r = 0.2368) and negatively correlated with pH, sand, SP, Na, K, P and N. There were obvious differences in correlation coefficients among the selected criteria (37% of the total number of correlation coefficients were positively correlated between bacterial counts and soil physico-chemical properties whereas 63% of the total number were positively correlated between plant type and bacterial counts). The formulation of an appropriate national strategy in biotechnology should constitute an important, initial step towards the utilization and industrialization of microorganisms. The development of molecular techniques of microbial identification, coupled with traditional methods is promising areas for continued research.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Hassan, H. and El-Kamali, H. (2015) Effect of Soil Physico-Chemical Properties and Plant Type on Bacterial Diversity in Semi-Arid Parts in Central Sudan. Part I: Omdurman North Region. Open Access Library Journal, 2, 1-9. doi: 10.4236/oalib.1101863.

References

[1] Giller, K.E., Beare, M.H., Lavelle, R. and Izac, A. (1997) Agricultural Intensification. Soil Biodiversity and Agroecosystem Function. Applied Soil Ecology, 6, 3-16.
http://dx.doi.org/10.1016/S0929-1393(96)00149-7
[2] Dick, R.P. (1994) Soil Enzyme Activities as Indicators of Soil Quality. In: Doran, J.W., Coleman, D.C., Bezdicek, D.F. and Stewart, B.A., Eds., Defining Soil Quality for a Sustainable Environment, Soil Science Society of America.
[3] Cheesbrough, M. (1984) Culture Media. In: Medical Laboratory Manual for Tropical Countries, Tropical Health Technology and Butterworth-Heineman, Cambridge, Vol. III.
[4] Cruickshank, R. (1975) Medical Microbiology: A Guide to Diagnosis and Control of Infection. E and S Livingston Ltd., Edinburgh and London, 888.
[5] Barrow, G.H. and Feltham, R.K.A. (1993) Cowan and Steel’s Manual for Identification of Medical Bacteria. 3rd Edition, Cambridge University Press, Cambridge, 331.
http://dx.doi.org/10.1017/CBO9780511527104
[6] Williams, S.T. and Cross, T. (1971) Actinomycetes. In: Booth, C., Eds., Methods in Microbiology, Academic Press, London.
[7] Collins, C.H., Lyne, P.M. and Grange, J. (1995) Collins and Layne’s Microbiological Methods. Butterworth-Heinemann, London.
[8] Benson, T. (2001) Microbiological Applications Laboratory Manual in General Microbiology. 8th Edition, The McGraw-Hill, New York.
[9] You, K.M. and Park, Y.K. (2004) A New Method for the Selective Isolation of Actinomycetes from Soil. Biotechnology Techniques, 10, 541-546.
[10] Richards, L.A. (1954) Diagnosis and Improvement of Saline and Alkali Soils. US Dept. Agr. Handbook 60 US Gov. Printing Office, Washington DC.
[11] Moghimi, A.H., Hamdan, J., Shamshuddin, J., Samsuri, A.W. and Abtahi, A. (2013) Physicochemical Properties and Surface Charge Characteristics of Arid Soils in Southern Iran. Applied and Environmental Soil Science, 2013, Article ID: 252861.
http://dx.doi.org/10.1155/2013/252861
[12] Subrahmanyam, N.S. and Sambamurty, A.V.S.S. (2000) Ecology. Narosa Publishing House, New Delhi, 172.
[13] Atlas, R.M. (1984) Diversity of Microbial Communities. In: Marshall KC., Ed., Advances in Microbial Ecology, Plerum Press, New York, 1-47.
http://dx.doi.org/10.1007/978-1-4684-8989-7_1
[14] Turco, R.F., Kennedy, A.C. and Jawson, M.D. (1994) Microbial Indicators of Soil Quality. In: Doran, J.W., Coleman, D.C., Bezdicek, D.F. and Stewart, B.A., Eds., Defining Soil Quality for a Sustainable Environment, Soil Science Society of America Special Publication No. 35, SSSA, Madison, 73-90.
[15] McCulley, R.L. and Burke, L.C. (2004) Microbial Community Composition across the Great Plains: Landscape versus Regional Variability. Soil Science Society of America Journal, 68, 106-115.
http://dx.doi.org/10.2136/sssaj2004.1060
[16] Garbeva, P., van Veen, J.A. and Elsas, J.D. (2004) Microbial Diversity in Soil: Selection of Microbial Populations by Plant and Soil Type and Implications for Disease Suppressiveness. Annual Review of Phytopathology, 42, 243-270.
http://dx.doi.org/10.1146/annurev.phyto.42.012604.135455
[17] Fang, M., Kremer, R.J., Motavalli, P.P. and Davis, G. (2005) Bacteria Diversity in Rhizospheres of Nontransgenic and Transgenic Corn. Applied and Environmental Microbiology, 71, 4132-4136.
http://dx.doi.org/10.1128/AEM.71.7.4132-4136.2005
[18] Carney, K.M. and Matsu, P.A. (2005) Plant Communities, Soil Microorganisms, and Soil Carbon Cycling: Does Altering the World Belowground Matter to Ecosystem Functioning? Ecosystems, 8, 928-940.
http://dx.doi.org/10.1007/s10021-005-0047-0
[19] Heritage, J., Evans, E. and Killington, R. (2003) Microbiology in Action. Cambridge University Press, Cambridge.
[20] Silver, W., Neff, J., McGroddy, M., Veldkamp, E., Keller, M. and Cosme, R. (2000) Effects of Soil Texture on Belowground Carbon and Nutrient Storage in Lowland. Amazonian Forest Ecosystem. Ecosystems, 3, 193-209.
http://dx.doi.org/10.1007/s100210000019
[21] Matus, F.J., Christopher, H.L. and Christian, R.M. (2008) Effect of Soil Texture, Carbon Input Rates, and Litter Quality on Free Organic Matter and Nitrogen Mineralization in Chilean Rain Forest and Agricultural Soils. Communications in Soil Science and Plant Analysis, 39, 187-201.
http://dx.doi.org/10.1080/00103620701759137
[22] Raiesi, F. (2006) Carbon and N Mineralization as Affected by Soil Cultivation and Crop Residue in a Calcareous Wetland Ecosystem in Central Iran. Agriculture, Ecosystems & Environment, 112, 13-20.
http://dx.doi.org/10.1016/j.agee.2005.07.002
[23] Ndaw, S.M., Gama-Rodrigues, A.C., Gama-Rodrigues, E.F., Sales, K.R. and Rosado, A.S. (2009) Relationship between Bacterial Diversity, Microbial Biomass, and Litter Quality in Soils under Different Plant Covers in Northern Rio de Janeiro State, Brazil. Canadian Journal of Microbiology, 55, 1089-1095.
http://dx.doi.org/10.1139/W09-066
[24] Stein, T. (2005) Bacillus subtilis Antibiotics: Structures, Synthesis and Specific Functions. Molecular Microbiology, 56, 845-857.
http://dx.doi.org/10.1111/j.1365-2958.2005.04587.x
[25] Demain, A.L. and Fang, A. (2000) The Natural Functions of Secondary Metabolites. In: Fiechter, A., Ed., History of Modern Biotechnology I, Advances in Biochemical Engineering/Biotechnology, Vol. 69, Springer, Berlin, 1-39.
http://dx.doi.org/10.1007/3-540-44964-7_1
[26] Sansinenea, E. and Ortiz, A. (2011) Secondary Metabolites of Soil Bacillus Spp. Biotechnology Letters, 33, 1523-1538.
http://dx.doi.org/10.1007/s10529-011-0617-5

  
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