Isolation and Characterization of Novel Microsatellite Markers in Chayote [Sechium edule (Jacq.) Sw.]


Chayote [Sechium edule (Jacq.) Sw.] is an economically important species in Latin America; however, there are very few reports available regarding its genetic diversity. Out of 11 microsatellite markers isolated, 10 loci provided 1 to 7 alleles per locus in a set of Mexican chayote accessions. Observed and expected heterozygosities for each locus ranged from 0.00 to 0.85 and 0.00 to 0.73, respectively. The overall genetic diversity detected by microsatellites was compared with that detected by P450-based analogue markers, a genome-wide dominant marker. Genetic diversity values obtained by the newly designed microsatellite markers were almost equal to the value estimated by PBA markers, but genetic distances calculated by both marker systems were not significantly correlated. Additional microsatellite markers, which could detect more polymorphisms, may be necessary to analyze the genetic diversity and structure of Mexican chayote collections.

Share and Cite:

Machida-Hirano, R. , Cortés-Cruz, M. , Amaro González, B. , Íñiguez, J. , Shirata, K. and Watanabe, K. (2015) Isolation and Characterization of Novel Microsatellite Markers in Chayote [Sechium edule (Jacq.) Sw.]. American Journal of Plant Sciences, 6, 2033-2041. doi: 10.4236/ajps.2015.613203.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Avendaño-Arrazate, C.H., Cadena-íñiguez, J., Arévalo-Galarza, M.L., Campos-Rojas, E., Cisneros-Solano, V.M. and Aguirre-Medina, J.F. (2010) Las variedades del chayote Mexicano, recurso ancestral con potencial de comercialización. Grupo Interdisciplinario de Investigación en Sechium edule en México A.C. (GISeM), Mexico (in Spanish).
[2] Cadena-Iñiguez, J., Arévalo-Galarza, L., Avendaño-Arrazate, C., SotoHernández, M., Ruiz-Posadas, L., Santiago-Osorio, E., Acosta-Ramos, M., Cisneros-Solano, V., Aguirre-Medina, J. and Ochoa-Martínez, D. (2007) Production, Genetics, Postharvest Management and Pharmacological Characteristics of Sechium edule (Jacq.) Sw. In: Teixeira da Silva, J., Ed., Fresh Produce,Vol. 1, Global Science Books, Middlesex, 41-53.
[3] Flores, M.E. (1989) El Chayote, Sechium edule Swartz (Cucurbitaceae). Revista de Biología Tropical, 37, 1-54 (in Spanish).
[4] Cadena-Iñiguez, J., Ruiz-Posadas, L.M., Trejo-López, C., Sánchez-García, P. and Aguirre-Medina, J.F. (2001) Intercambio de gases y relaciones hídricas del chayote (Sechium edule (Jacq.) Sw). Revista Chapingo, 7, 21-35 (in Spanish).
[5] Cadena-Iñiguez, J. and Arévalo-Galarza, M.L.C. (2011) Las variedades del chayote (Sechium edule (Jacq) Sw.) y su comercio mundial. Primera edición Mundi Prensa México (in Spanish).
[6] Lira, R. (1995) Estudios taxonómicos y ecogeográficos de las Cucurbitáceas latinoamericanas de importancia económica. Systematic and ecogeographic studies on crop genepools. 9. International Plant Genetic Resources Institute, Rome (in Spanish).
[7] Newstrom, L.E. (1990) Origin and Evolution of Chayote. In: Bates, D.M., Robinson, R.W. and Jeffrey, C., Eds., Biology and Utilization of the Cucurbitaceae, Cornell University Press, Ithaca, New York, 141-149.
[8] Bates, M.D., Merrick, L.C. and Robinson, R.W. (1995) 24 Minor Cucurbits. In: Smartt, J. and Simmonds, N.W., Eds., Evolution of Crop Plants, 2nd Edition, Wiley-Blackwell, New York, 105-111.
[9] Lira, S.R. (1996) Chayote. Sechium edule (Jacq.) Sw. Promoting the Conservation and Use of Underutilized and Neglected Crops. 8. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome.
[10] Cadena-íñiguez, J., Avendaño-Arrazate, C.H., Soto-Hernández, M., Ruiz-Posadas, L.M., Aguirre-Medina, J.F. and Arévalo-Galarza, L. (2008) Infraspecic Variation of Sechium edule (Jacq.) Sw. in the State of Veracruz, Mexico. Genetic Resources and Crop Evolution, 55, 835-847.
[11] Abdelnour, A. and Rocha, O.J. (2008) Genetic Characterization of a Collection of Chayote, Sechium edule (Jacq.) Swartz, in Costa Rica by Using Isozyme Markers. Genetic Resources and Crop Evolution, 55, 163-170.
[12] Avendaño-Arrazate, C.H., Cadena-íñiguez, J., Arévalo-Galarza, M.L., Cisneros-Solano, V.M., Aguirre-Medina, J.F., Moreno-Pérez, E.D.C., Cortés-Cruz, M., Castillo-Martínez, C.R. and Ramírez Vallejo, P. (2012) Variación genética en el complejo infraespecífico de chayote evaluada mediante sistemas isoenzimáticos. Pesquisa Agropecuária Brasileira, 47, 244-252 (in Spanish).
[13] Cross, H., Lira Saade, R. and Motley, T.J. (2006) Origin and Diversification of Chayote. In: Motley, T.J., Zerega, N. and Cross, N., Eds., Darwin’s Harvest, Columbia University Press, New York, 171-194.
[14] Nybom, H., Weising, K. and Rotter, B. (2014) DNA Fingerprinting in Botany: Past, Present and Future. Investigative Genetics, 5, 1.
[15] Yamanaka, S., Suzuki, E., Tanaka, M., Takeda, Y., Watanabe, J.A. and Watanabe, K.N. (2003) Assessment of Cytochrome P450 Sequences Offers a Useful Tool for Determining Genetic Diversity in Higher Plant Species. Theoretical and Applied Genetics, 108, 1-9.
[16] Gilani, S.A., Kikuchi, A. and Watanabe, K.N. (2009) Genetic Variation within and among Fragmented Populations of Endangered Medicinal Plant, Withania coagulans (Solanaceae) from Pakistan and Its Implications for Conservation. African Journal of Biotechnology, 8, 2948-2958.
[17] Jatoi, S.A., Kikuchi, A., Ahmad, D. and Watanabe, K.N. (2010) Characterization of the Genetic Structure of Mango Ginger (Curcuma amada Roxb.) from Myanmar in Farm and Genebank Collection by the Neutral and Functional Genomic Markers. Electronic Journal of Biotechnology, 13, 1-11.
[18] Yamanaka, S., Ikeda, S., Imai, A., Luan, Y., Watanabe, J.A. and Watanabe, K.N. (2005) Construction of Integrated Genetic Map between Various Existing DNA Markers and Newly Developed P450-Related PBA Markers in Diploid Potato (Solanum tuberosum). Breeding Science, 55, 223-230.
[19] Yamanaka, S., Jatoi, S.A., San-Yi, S., Kothari, S.L. and Htut, T. and Watanabe, K.N. (2011) Genetic Diversity of Myanmar Rice and Their Implementation on Management Methods. African Journal of Biotechnology, 10, 1290-1298.
[20] Doyle, J.J. and Doyle, J.L. (1987) A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19, 11-15.
[21] Lian, C. and Hogetsu, T. (2002) Development of Microsatellite Markers in Black Locust (Robinia pseudoacacia) Using a Dual-Suppression-PCR Technique. Molecular Ecology Notes, 2, 211-213.
[22] Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R. and Leunissen, J.A.M. (2007) Primer3Plus, an Enhanced Web Interface to Primer3. Nucleic Acids Research, 35, W71-W74.
[23] Blacket, M.J., Robin, C., Good, R.T., Lee, S.F. and Miller, A.D. (2012) Universal Primers for Fluorescent Labelling of PCR Fragments—An Efficient and Cost-Effective Approach to Genotyping by Fluorescence. Molecular Ecology Resources, 12, 456-463.
[24] Peakall, R. and Smouse, P.E. (2012) GenAlEx 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research—An Update. Bioinformatics, 28, 2537-2539.
[25] Kemp, S. (2002) PIC Calculator Extra.
[26] Mantel, N. (1967) The Detection of Disease Clustering and a Generalized Regression Approach. Cancer Research, 27, 209-220.
[27] Côrtes, M., Gowda, V., Kress, W., Bruna, E.M. and Uriarte, M. (2009) Characterization of 10 Microsatellite Markers for the Understorey Amazonian Herb Heliconia acuminata. Molecular Ecology Resources, 9, 1261-1264.
[28] Katzir, N., Danin-Poleg, Y., Tzuri, G., Karchi, Z., Lavi, U. and Cregan, P.B. (1996) Length Polymorphism and Homologies of Microsatellites in Several Cucurbitaceae Species. Theoretical and Applied Genetics, 93, 1282-1290.
[29] Danin-Poleg, Y., Reis, N., Tzuri, G. and Katzir, N. (2001) Development and Characterization of Microsatellite Markers in Cucumis. Theoretical and Applied Genetics, 102, 61-72.
[30] Watcharawongpaiboon, N. and Chunwongse, J. (2008) Development and Characterization of Microsatellite Markers from an Enriched Genomic Library of Cucumber (Cucumis sativus). Plant Breeding, 127, 74-81.
[31] Wang, S.Z., Pan, L., Hu, K., Chen, C.Y. and Ding, Y. (2010) Development and Characterization of Polymorphic Microsatellite Markers in Momordica charantia (Cucurbitaceae). American Journal of Botany, 97, e75-e78.
[32] Newstorm, L.E. (1985) Collection of Chayote and Its Wild Relatives. FAO/IBPGR Plant Genetic Resources Newsletter, 64, 14-20.
[33] Grubben, G.J.H., Denton, O.A., Messiaen, C.M., Schippers, R.R. and Lemmens, R.H.M.J. (2005) Vegetables. Backhuys Publishers, Wageningen, 2.
[34] Ritschel, P.S., de Lima Lins, T.C., Tristan, R.L., Cortopassi-Buso, G.S., Amauri-Buso, J. and Ferreira, M.E. (2004) Development of Microsatellite Markers from an Enriched Genomic Library for Genetic Analysis of Melon (Cucumis melo L.). BMC Plant Biology, 4, 9.
[35] Cadena-Iñiguez, J. (2005) Caracterización morfoestructural, fisiológica, química y genética de diferentes tipos de chayote (Sechium edule (Jacq.) Sw.). PhD Thesis, Colegio de Postgraduados, Texcoco (in Spanish).

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.