Dead Sea Minerals-Induced Positive Stress as an Innovative Resource for Skincare Actives

Meital Portugal-Cohen^1,2*, Maria F. Dominguez³, Miriam Oron^1,2, Robert Holtz⁴, Ze’evi Ma’or^1,2

¹Ahava-Dead Sea Laboratories, Lod, Israel.
²Dead Sea and Arava Science Center, The Laboratory for Skin Biochemistry and Biotechnology, Ein Gedi, Israel.
³Lonza-Personal Care, South Plainfield, USA.
⁴BioInnovation Laboratories Inc., Lakewood, USA.

DOI: 10.4236/jcdsa.2015.51004                

Abstract

Objective: Exposure to certain stresses in small doses might lead to a protective effect by improving resistance to other stressors. Dead Sea (DS) minerals can be a relevant source to induce positive stress due to their high salinity and unique mineral combination. This concept could be further optimized using advanced unique cell biotechnology. The purpose of this study was to elucidate the innovative concept of DS minerals (water extract and black mud) supplementation in small amount to Pichia pastoris yeast growth media as a positive stress by testing the capability of accepted fermentation compounds to affect the appearance of skin. Methods: Skin equivalents were topically applied with different Pichia pastoris fermentations (Metabiotics?). Skin elasticity biomarkers were tested, since loss of elasticity and suppleness is a natural skin aging process leading to deeper wrinkles and loss of firmness. A preliminary screening at the gene level using DNA microarray was performed and subsequently, the following proteins were detected using ELISA or immunoblotting assays: elastin, fibulin-1, lysyl oxidase (LOX), metalloproteinase 3 (MMP-3), E-cadherin, claudin 4, tight junction protein (TJP)-1 and TJP-2. UVB irradiation was selected as a stressor. Results: Fermentation compounds generated in the presence of small doses of DS minerals affected the expression of various elasticity-related genes in skin. Moreover, they significantly attenuated the abnormal UVB-induced alterations, the proteins elastin, fibulin-1, LOX, MMP-3, E-cadherin and TJP-2. Conclusions: The observations clearly demonstrate that when DS Metabiotics? compounds are topically applied, significant alterations in several biomarkers that contribute to skin elasticity occur. Thus, these novel compounds have the potential to serve as skincare actives.

Keywords

Skin Elasticity Biomarkers, Skin Aging, Active Ingredients, Dead Sea Minerals, Positive Stress

Conflicts of Interest

The authors declare no conflicts of interest.

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