Quantitative Estimation of Gallic Acid as Biomarker in Lipitame Tablets by HPTLC Densitometry for Diabetic Dyslipidemia

Sheeraz Siddiqui¹, Khan Usmanghani^1,2, Aqib Zahoor², Zeeshan Ahmed Sheikh², Saleha Suleman Khan²

¹Faculty of Eastern Medicine, Hamdard University, Karachi, Pakistan.
²Research and Development Department, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan.

DOI: 10.4236/cm.2014.54021                

Abstract

Lipitame is a poly herbal formulation comprised of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica. The formulation is investigated for its analysis evaluation. Biomarkers of three herbs such as Terminalia arjuna, Terminalia belerica, and Phyllanthus emblica have been already cited to contain gallic acid, which was qualitatively and quantitatively estimated. In the present study rapid and inexpensive qualification methods for the quality control of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica on thin layer chromatography (TLC) were developed and validated. The solvent system used was toluene:ethyl acetate:formic acid:methanol (12:9:4:0.5). The scanning of plate was performed linearly at 273 nm (absorption) by use of a TLC Scanner III CAMAG with a deuterium source, and the area of spots corresponding to Gallic acid standard was integrated. It was found that gallic acid has been found in the Lipitame tablets on HPTLC densitometry assessment compared with authentic gallic acid reference standard.

Keywords

Lipitame, Tablets, Quantitative Estimation, Densitometry, Diabetic Dyslipidemia

Conflicts of Interest

The authors declare no conflicts of interest.

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