In Vitro Evaluations for a New Topical Anti-Aging Formulation


The study aimed to evaluate the in vitro properties of a new gel formulation (P-3086) as anti-aging treatment. Two in vitro methods aimed to assess and compare the efficacy of the gel formulation in reducing oxidative damages, artificially induced by UVA on skin-derived keratinocytes and in promoting the synthesis of collagen, compared with other four formulations. P-3086 reduced reactive oxygen species production after UVA stress with the highest effect observed at 0.016 mg/ml and 0.031 mg/ml concentration. P-3086 also promoted the collagen synthesis faster when compared with other formulations. The new gel product, based on hyaluronic acid, Vitamin E and Humulus lupulus, showed a good efficacy as anti-aging effect reducing the oxidative damages derived by the action of ROS, moreover stimulating the synthesis of one of the components of the connective tissue, the collagen.

Share and Cite:

Scarci, F. and Mailland, F. (2014) In Vitro Evaluations for a New Topical Anti-Aging Formulation. Journal of Cosmetics, Dermatological Sciences and Applications, 4, 316-322. doi: 10.4236/jcdsa.2014.45041.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Panas, M., et al. (2012) The Byzantine Empress Zoe Porphyrogenita and the Quest for Eternal Youth. Journal of Cosmetic Dermatology, 11, 245-248.
[2] Datta, H.S., et al. (2011) Theories and Management of Aging: Modern and Ayurveda Perspectives. Evidence-Based Complementary and Alternative Medicine, 2011, Article ID 528527, 6 p.
[3] Park, H.M., et al. (2012) Royal Jelly Increases Collagen Production in Rat Skin after Ovariectomy. Journal of Medicinal Food, 15, 568-575.
[4] Lee, N.J., et al. (2011) In Vitro Antioxidant Properties of a Ginseng Intestinal Metabolite IH-901. Laboratory Animal Research, 27, 227-234.
[5] Khansari, N., Shakiba, Y. and Mahmoudi, M. (2009) Chronic Inflammation and Oxidative Stress as a Major Cause of Age-Related Diseases and Cancer. Recent Patents on Inflammation & Allergy Drug Discovery, 3, 73-80.
[6] Orrenius, S. (2007) Reactive Oxygen Species in Mitochondria-Mediated Cell Death. Drug Metabolism Reviews, 39, 443-455.
[7] Fisher, G.J. (2005) The Pathophysiology of Photoaging of the Skin. Cutis, 75, 5-9.
[8] Stefanaki, C., et al. (2005) Topical Retinoids in the Treatment of Photoaging. Journal of Cosmetic Dermatology, 4, 130-134.
[9] Philips, N., et al. (2010) Direct Inhibition of Elastase and Matrixmetalloproteinases and Stimulation of Biosynthesis of Fibrillar Collagens, Elastin, and Fibrillins by Xanthohumol. Journal of Cosmetic Science, 61, 125-132.
[10] Chevallier, A. (1996) The Encyclopedia of Medicinal Plants: An Excellent Guide to over 500 of the More Well-Known Medicinal Herbs from around the World. Dorling Kindersley, London.
[11] Kanthasamy, A., et al. (1997) Reactive Oxygen Species Generated by Cyanide Mediate Toxicity in Rat Pheochromocytoma Cells. Toxicology Letters, 93, 47-54.
[12] Mosmann, T. (1983) Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Journal of Immunological Methods, 65, 55-63.
[13] Sparavigna, A., et al. (2014) An Innovative Concept Gel to Prevent Skin Aging. Journal of Cosmetics, Dermatological Sciences and Applications, 3, 4.
[14] Kato, S., et al. (2012) Hydrogen-Rich Electrolyzed Warm Water Represses Wrinkle Formation Against UVA Ray Together with Type-I Collagen Production and Oxidative-Stress Diminishment in Fibroblasts and Cell-Injury Prevention in Keratinocytes. Journal of Photochemistry and Photobiology B: Biology, 5, 24-33.
[15] Vedamurthy, M. (2006) Antiaging Therapies. Indian Journal of Dermatology, Venereology and Leprology, 72, 183-186.

Copyright © 2022 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.