Systemic Activation of Defensive Enzymes and Protection in Tobacco Plantlets against Phytophthora nicotianae Induced by Oligosaccharins

Alejandro Bernardo Falcón-Rodríguez¹, Daimy Costales¹, Juan Carlos Cabrera², Ruddy Wattiez³, Miguel Ángel Martínez-Téllez⁴

¹Group of Bioactive Products, Department of Plant Physiology and Biochemistry, National Institute of Agricultural Science, Mayabeque, Cuba.
²Unit of Biotechnology, Materia-Nova, Ghislenghiem, Belgium.
³Institute of Biosciences, University of Mons, Mons, Belgium.
⁴Center for Food Research and Development (CIAD), Coordination of Technology of Foods from Plant Origin, Hermosillo, Mexico.

DOI: 10.4236/ajps.2014.521351                

Abstract

Oligosaccharins are potent biomolecules which activate defense responses and resistance in tobacco plants. However, it is not known the systemic behavior of defensive enzymes activated by these elicitors. In this work, the dynamic behavior of key defensive enzymes was evaluated in tobacco plant leaves previously treated through the roots with chitosan polymer (CH), chitosan (COS) and pectic (OGAS) oligosaccharides and Spermine (Sp). All macromolecules tested activated protein levels and defense enzymatic activity in tobacco leaves but with different response dynamics among them and depending on the biochemical variable evaluated. Defense response above control levels were detected since 12 hours after treatments and it consisted in a biphasic behavior with two peaks for PAL (EC 4.3.1.5) and β 1 - 3 glucanase (EC 3.2.1.6) enzymatic activities. The highest enzymatic levels for these enzymes were achieved at 48 hours in plantlets elicited with COS and at 72 hours for those plants treated with chitosan polymer, while the highest POD (EC 1.11.1.6) activity was detected with CH between 48 and 72 hours. These results demonstrated systemic defense activation by oligosaccharins in tobacco whose dynamic of defense response is affected by the kind of oligosaccharins tested. When applying OGAS by foliar spray on tobacco, systemic resistance against Phytoththora nicotianae was induced and plantlets were protected with the low concentration tested by 46% under the bioassays conditions performed. Moreover, enzymatic determinations on roots and leaves previous to plant-pathogen interaction showed increments above 30% of control levels for PAL and POD activities. It means that oligosaccharins activate local and systemic defense responses in plants in the absent of pathogen infection.

Keywords

Systemic Induced Resistance, Oligosaccharins, Tobacco, Defensive Enzymes

Conflicts of Interest

The authors declare no conflicts of interest.

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