Systemic Activation of Defensive Enzymes and Protection in Tobacco Plantlets against Phytophthora nicotianae Induced by Oligosaccharins


Oligosaccharins are potent biomolecules which activate defense responses and resistance in tobacco plants. However, it is not known the systemic behavior of defensive enzymes activated by these elicitors. In this work, the dynamic behavior of key defensive enzymes was evaluated in tobacco plant leaves previously treated through the roots with chitosan polymer (CH), chitosan (COS) and pectic (OGAS) oligosaccharides and Spermine (Sp). All macromolecules tested activated protein levels and defense enzymatic activity in tobacco leaves but with different response dynamics among them and depending on the biochemical variable evaluated. Defense response above control levels were detected since 12 hours after treatments and it consisted in a biphasic behavior with two peaks for PAL (EC and β 1 - 3 glucanase (EC enzymatic activities. The highest enzymatic levels for these enzymes were achieved at 48 hours in plantlets elicited with COS and at 72 hours for those plants treated with chitosan polymer, while the highest POD (EC activity was detected with CH between 48 and 72 hours. These results demonstrated systemic defense activation by oligosaccharins in tobacco whose dynamic of defense response is affected by the kind of oligosaccharins tested. When applying OGAS by foliar spray on tobacco, systemic resistance against Phytoththora nicotianae was induced and plantlets were protected with the low concentration tested by 46% under the bioassays conditions performed. Moreover, enzymatic determinations on roots and leaves previous to plant-pathogen interaction showed increments above 30% of control levels for PAL and POD activities. It means that oligosaccharins activate local and systemic defense responses in plants in the absent of pathogen infection.

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Falcón-Rodríguez, A. , Costales, D. , Cabrera, J. , Wattiez, R. and Martínez-Téllez, M. (2014) Systemic Activation of Defensive Enzymes and Protection in Tobacco Plantlets against Phytophthora nicotianae Induced by Oligosaccharins. American Journal of Plant Sciences, 5, 3354-3363. doi: 10.4236/ajps.2014.521351.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Esquerré-Tugayé, M.-T., Boudart, G. and Dumas, B. (2000) Cell Wall Degrading Enzymes, Inhibitory Proteins and Oligosaccharides Participate in the Molecular Dialogue between Plants and Pathogen. Plant Physiology and Biochemistry, 38, 157-163.
[2] Vorwerk, S., Somerville, S. and Somerville, C. (2004) The Role of Plant Cell Wall Polysaccharide Composition in Plant Disease Resistance. Trends Plant Sciences, 9, 203-209.
[3] Courtois, J. (2009) Oligosaccharides from Land Plants and Algae: Production and Applications in Therapeutics and Biotechnology. Current Opinion in Microbiology, 12, 261-273.
[4] Ridley, B.L., O’Neill, M.A. and Mohnen, D. (2001) Pectins: Structure, Biosynthesis, and Oligogalacturonide-Related Signaling. Phytochemistry, 57, 929-967.
[5] Shibuya, N. and Minami, E. (2001) Oligosaccharide Signalling for Defense Responses in Plant. Physiological and Molecular Plant Pathology, 59, 223-233.
[6] Aziz, A., Heyraud, A. and Lambert, B. (2004) Oligogalacturonide Signal Transduction, Induction of Defense-Related Responses and Protection of Grapevine against Botrytis cinerea. Planta, 218, 767-774.
[7] Falcón-Rodríguez, A.B., Costales-Menéndez, D., Ortega-Delgado, E., León-Díaz, O., Cabrera-Pino, J.C. and Martínez-Téllez, M.A. (2007) Evaluation of Chitosan as Inhibitor of Soilborne Pathogens and Elicitor of Defence Markers and Resistance in Tobacco Plants. Spanish Journal of Agricultural Research, 5, 533-541.
[8] Falcón-Rodríguez, A.B., Cabrera, J.C., Ortega, E. and Martínez-Téllez, M.A. (2009) Concentration and Physico-Chemical Properties of Chitosan Derivatives Determine the Induction of Defense Responses in Roots and Leaves of Tobacco (Nicotianatabacum) Plants. American Journal of Agricultural and Biological Sciences, 4, 192-200.
[9] Falcón-Rodríguez, A.B., Costales, D., Cabrera, J.C. and Martínez-Téllez, M.A. (2011) Chitosan Physic-Chemical Properties Modulate Defense Responses and Resistance in Tobacco Plants against the Oomycete Phytophthora nicotianae. Pesticide Biochemistry and Physiology, 100, 221-228.
[10] Cabrera, J.C., Gómez, R., Diosdado, E., Hormaza, J., Iglesias, R. Gutiérrez, A. and González, S. (2003) Procedure to Obtain a Pectic Oligosaccharide Mixture with Plant Rooting Activity. Cuban Patent No. 22859, Resolution 155/2003.
[11] Hoagland, D.R. and Arnon, D.I. (1950) The Water Culture Method of Growing Plants without Soil. California Agricultural Experimental Station, Circ 347.
[12] Falcón, A.B., Cabrera, J.C., Costales, D., Ramírez, M.A., Cabrera, G., Toledo, V. and Martínez-Téllez, M.A. (2008) The Effect of Size and Acetylation Degree of Chitosan Derivatives on Tobacco Plant Protection against Phytophthora parasitica nicotianae. World Journal Microbiology and Biotechnology, 24, 103-112.
[13] van Loon, L.C., Rep, M. and Pieterse, C.M.J. (2006) Significance of Inducible Defense-Related Proteins in Infected plants. Annual Review of Phytopathology, 44, 135-162.
[14] Garcia-Brugger, A., Lamotte, O., Vandelle, E., Bourque, S., Lecourieux, D., Poinssot, B., Wendehenne, D. and Pugin, A. (2006) Early Signaling Events Induced by Elicitors of Plant Defenses. Molecular Plant-Microbe Interaction, 19, 711-724.
[15] Desender, S., Andrivon, D. and Val, F. (2007) Activation of Defense Reactions in Solanaceae: Where Is the Specificity? Cellular Microbiology, 9, 21-30.
[16] Heil, M. and Bostock, R. (2002) Induced Systemic Resistance (ISR) against Pathogens in the Context of Induced Plant Defenses. Annual of Botany, 89, 503-512.
[17] Heil, M. and Walters, D. R. (2009) Ecological Consequences of Plant Defence Signalling. In: van Loon, L.C., Ed., Advances in Botanical Research, Vol. 51, Academic Press, Burlington, 667-716.
[18] Grenier, J. and Asselin, A. (1990) Some Pathogenesis-Related Proteins Are Chitosanases with Lytic Activity against Fungal Spores. Molecular Plant-Microbe Interaction, 3, 401-407.
[19] Vander, P., Varum, K.M., Domard, A., El Gueddari, N.E. and Moerschbacher, B.M. (1998) Comparison of the Ability of Partially N-Acetylated Chitosans and Chitooligosaccharides to Elicit Resistance Reactions in Wheat Leaves. Plant Physiology, 118, 1353-1359.

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