Anal Invasive Squamous Cell Cancer and Human Papillomavirus Distribution in HIV-Infected and Non-HIV-Infected Individuals
Laila Darwich1,2, Sebastián Videla1,3*, Mari-Paz Cañadas1,4, Josep Coll1,5, Boris Revollo1, Marta Piñol1, Francesc García-Cuyás1, Eva Castella1, Bonaventura Clotet1,5,6,7, Guillem Sirera1,5
1Lluita Contra La SIDA Foundation, University Hospital Germans Trias i Pujol, Badalona, Spain.
2Departament de Sanitat i d’Anatomia Animals, Faculty of Veterinary, Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.
3Department of Experimental and Health Sciences, Faculty of Health and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain.
4General-Labco, Barcelona, Spain.
5HIV Clinical Unit, University Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona (UAB), Badalona, Spain.
6Retrovirology Laboratory IrsiCaixa Foundation, University Hospital Germans Trias i Pujol, Badalona, Spain.
7Universitat de Vic, Vic, Spain.
DOI: 10.4236/ijcm.2014.519148   PDF   HTML     3,028 Downloads   3,626 Views   Citations


Invasive-squamous-cell-cancer (ISCC) of the anal canal is an uncommon disease. Human papillomavirus (HPV) is the etiological agent of most of types of ISCC. The incidence of ISCC has been increasing in HIV-infected individuals, even after the introduction of highly active antiretroviral therapy. The aim of this study was to analyze biopsy specimens from patients diagnosed with ISCC at a tertiary hospital from 1983 to 2012 in order to detect HPV-DNA. Methods: Formaldehyde-fixed, paraffin-embedded specimens from patients with ISCC underwent HPV-DNA genotyping using multiplex PCR assay. Results: A total of 31 cases were collected; 10 were HIV-infected (9 men, 1 woman) and 21 non-HIV-infected (11 men, 10 women). HPV infection was detected in 87.5% (7/8) of the HIV-infected patients (DNA from 2 biopsies was degraded) and 76.2% (16/21) of non-HIV-infected individuals. Multiple-type infections were only found in 28.6% (2/7) of the HIV-infected patients (no multiple-type infections in non-HIV-infected individuals). The most prevalent type was HPV-16: 50% (4/8) in the HIV-infected group (57% [4/7] of the HPV-positive samples) and 66.7% (14/21) in the non-HIV-infected group (87.5% (14/16) of the HPV-positive samples). Remarkably, 37.5% (3/8) of the HIV-infected group had high-risk HPV types not included in the vaccines (HPV-33, 51, 52, and 66) compared with 4.8% in the non-HIV-infected group (1/21, HPV-52). All cases of anal ISCC in HIV-infected patients were recorded in the highly active antiretroviral therapy era. Conclusion: HIV-infected patients presented anal ISCC with a higher proportion of high-risk HPV types not covered by the conventional vaccines than non-HIV-infected individuals.

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Darwich, L. , Videla, S. , Cañadas, M. , Coll, J. , Revollo, B. , Piñol, M. , García-Cuyás, F. , Castella, E. , Clotet, B. and Sirera, G. (2014) Anal Invasive Squamous Cell Cancer and Human Papillomavirus Distribution in HIV-Infected and Non-HIV-Infected Individuals. International Journal of Clinical Medicine, 5, 1155-1160. doi: 10.4236/ijcm.2014.519148.

Conflicts of Interest

The authors declare no conflicts of interest.


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