The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

Elham O. Mahgoub

Alneelain Medical Research Center, Faculty of Medicine Department of Microbiology and Unit of Immunology, Al-Neelain University, Khartoum, Sudan.

DOI: 10.4236/ojgen.2014.44029                

Abstract

Chicken anaemia virus (CAV) causes a viral disease in chickens worldwide and thus has economic importance. The main aim of this study was to develop a rapid, sensitive and specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1, was separately cloned and expressed in recombinant E. coli. The purified recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to evaluate its reactivity against chicken sera. The resulting indirect ELISA was then compared with a commercial ELISA. The specificity and sensitivity of the indirect ELISA were measured as 93.3% and 100%, respectively. A t-test produced a t-value of 15.805 for the indirect ELISA and revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.001). For the second variable (i.e., a commercial ELISA), the t-test yielded a t-value of 5.063, which revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention produces statistically significant improvements in both variables (p-values < 0.05). The correlation coefficient for the indirect ELISA was r = 0.93. Therefore, this work can be considered as a new achievement in diagnosis for Chicken anaemia virus in chicken flocks.

Keywords

Indirect ELISA, Commercial ELISA, Chicken Anaemia Virus, Recombinant Protein VP1, Sensitivity, Specificity, Standard Curve for Indirect ELISA

Conflicts of Interest

The authors declare no conflicts of interest.

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