Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires


Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5’ AAT GGA TTT ATC TGC TCT TCG 3’) specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00; as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 μM with a detection limit of 3.32 μM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.

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Mansor, N. , Zain, Z. , Hamzah, H. , Noorden, M. , Jaapar, S. , Beni, V. and Ibupoto, Z. (2014) Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires. Open Journal of Applied Biosensor, 3, 9-17. doi: 10.4236/ojab.2014.32002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Li, T., Fan, Q., Liu, T., Zhu, X., Zhao, J. and Li, G. (2010) Detection of Breast Cancer Cells Specially and Accurately by an Electrochemical Method. Biosensors and Bioelectronics, 25, 2686-2689.
[2] Dolatabadi, J.E.N., Mashinchian, O., Ayoubi, B., Jamali, A.A., Mobed, A., Losic, D., et al. (2011) Optical and Electrochemical DNA Nanobiosensors. TrAC Trends in Analytical Chemistry, 30, 459-472.
[3] Antoniou, A., Pharoah, P.D.P., Narod, S., Risch, H.A., Eyfjord, J.E., Hopper, J.L., et al., (2003) Average Risks of Breast and Ovarian Cancer Associated with BRCA1 or BRCA2 Mutations Detected in Case Series Unselected for Family History: A Combined Analysis of 22 Studies. The American Journal of Human Genetics, 72, 1117-1130.
[4] Li, C.-Z., Karadeniz, H., Canavar, E. and Erdem, A. (2012) Electrochemical Sensing of Label Free DNA Hybridization Related to Breast Cancer 1 Gene at Disposable Sensor Platforms Modified with Single Walled Carbon Nanotubes. Electrochimica Acta, 82, 137-142.
[5] Kamb, A., Futreal, P.A., Rosenthal, J., Cochran, C., Harshman, K.D., Liu, Q., et al., (1994) Localization of the VHR Phosphatase Gene and Its Analysis as a Candidate for BRCA1. Genomics, 23, 163-167.
[6] Culha, M., Stokes, D.L., Griffin, G.D. and Vo-Dinh, T. (2004) Application of a Miniature Biochip Using the Molecular Beacon Probe in Breast Cancer Gene BRCA1 Detection. Biosensors and Bioelectronics, 19, 1007-1012.
[7] Tiwari, A. and Gong, S. (2009) Electrochemical Detection of a Breast Cancer Susceptible Gene Using cDNA Immobilized Chitosan-co-Polyaniline Electrode. Talanta, 77, 1217-1222.
[8] Yin, H., Xu, Z., Wang, M., Zhang, X. and Ai, S. (2013) An Electrochemical Biosensor for Assay of DNA Methyltransferase activity and screening of inhibitor. Electrochimica Acta, 89, 530-536.
[9] Elzouki, A.-N., El-Menyar, A., Ahmed, E., Elbadri, M.E., Imam, Y.Z. and Gurbanna, B.A. (2010) Terlipressin-Induced Severe Left and Right Ventricular Dysfunction in Patient Presented with Upper Gastrointestinal Bleeding: Case Report and Literature Review. The American Journal of Emergency Medicine, 28, 540.e1-540.
[10] Wang, J. (2006) Electrochemical Biosensors: Towards Point-of-Care Cancer Diagnostics. Biosensors and Bioelectronics, 21, 1887-1892.
[11] Zhong, H., Yuan, R., Chai, Y., Li, W., Zhong, X. and Zhang, Y. (2011) In Situ Chemo-Synthesized Multi-Wall Carbon Nanotube-Conductive Polyaniline Nanocomposites: Characterization and Application for a Glucose Amperometric Biosensor. Talanta, 85, 104-111.
[12] Robles, T.F., Shetty, V., Zigler, C.M., Glover, D.A., Elashoff, D., Murphy, D. et al. (2011) The Feasibility of Ambulatory Biosensor Measurement of Salivary Alpha Amylase: Relationships with Self-Reported and Naturalistic Psychological Stress. Biological Psychology, 86, 50-56.
[13] Li, W., Yuan, R., Chai, Y., Zhong, H. and Wang, Y. (2011) Study of the Biosensor Based on Platinum Nanoparticles Supported on Carbon Nanotubes and Sugar-Lectin Biospecific Interactions for the Determination of Glucose. Electrochimica Acta, 56, 4203-4208.
[14] Zheng, B., Xie, S., Qian, L., Yuan, H., Xiao, D. and Choi, M.M.F. (2011) Gold Nanoparticles-Coated Eggshell Membrane with Immobilized Glucose Oxidase for Fabrication of Glucose Biosensor. Sensors and Actuators B: Chemical, 152, 49-55.
[15] Black, D. (1994) Familial Breast Cancer: BRCA1 down, BRCA2 to Go. Current Biology, 4, 1023-1024.
[16] Kumar, S.A., Cheng, H.W. and Chen, S.M. (2009) Electroanalysis of Ascorbic Acid (Vitamin C) Using Nano-ZnO/ Poly(Luminol) Hybrid Film Modified Electrode. Reactive and Functional Polymers, 69, 364-370.
[17] Jiang, L.C. and Zhang, W.D. (2009) A Highly Sensitive Nonenzymatic Glucose Sensor Based on CuO Nanoparticles- Modified Carbon Nanotube Electrode. Biosensors and Bioelectronics, 25, 1402-1407.
[18] Kong, T., Chen, Y., Ye, Y., Zhang, K., Wang, Z. and Wang, X. (2010) An Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the ZnO Nanotubes. Sensors and Actuators B: Chemical, 138, 344-350.
[19] Kilic, T., Topkaya, S.N., Ariksoysal, D.O., Ozsoz, M., Ballar, P., Erac Y., et al. (2012) Electrochemical Based Detection of MicroRNA, Mir21 in Breast Cancer Cells. Biosensors and Bioelectronics, 38, 195-201.
[20] Xu, X., Weng, X., Liu, A., Zhang, X., Wang, C., Chen, W., et al. (2013) An Electrochemical DNA Sensor for Detection of Cytokeratin 19. Analytical Methods, 5, 2329-2334.
[21] Ali, S.M.U., Ibupoto, Z.H., Salman, S., Nur, O., Willander, M. and Danielsson, B. (2011) Selective Determination of Urea Using Urease Immobilized on ZnO Nanowires. Sensors and Actuators B: Chemical, 160, 637-643.
[22] Ali, S.M.U., Alvi, N.H., Ibupoto, Z., Nur, O., Willander, M. and Danielsson, B. (2011) Selective Potentiometric Determination of Uric Acid with Uricase Immobilized on ZnO Nanowires. Sensors and Actuators B: Chemical, 152, 241-247.
[23] Chu, H.W., Thangamuthu, R. and Chen, S.M. (2007) Zinc Oxide/Zinc Hexacyanoferrate Hybrid Film-Modified Electrodes for Guanine Detection. Electroanalysis, 19, 1944-1951.
[24] Ye, Y. and Ju, H. (2005) Rapid Detection of ssDNA and RNA Using Multi-Walled Carbon Nanotubes Modified Screen-Printed Carbon Electrode. Biosensors and Bioelectronics, 21, 735-741.
[25] Souza, E., Nascimento, G., Santana, N., Ferreira, D., Lima, M., Natividade, E., et al. (2011) Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes. Sensors, 11, 5616-5629.

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