Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (Lupinus albus L.) Represent a Unique Genepool

Rosy Raman, Raymond B. Cowley, Harsh Raman, David J. Luckett

Graham Centre for Agricultural Innovation (an Alliance between Charles Sturt University and NSW Department of Primary Industries), NSW DPI, Agricultural Institute, Wagga Wagga, Australia.

DOI: 10.4236/ojgen.2014.42012                

Abstract

PCR-based genic and microarray-based Diversity Arrays Technology (DArT^TM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.

Keywords

Broad-Leaf Lupin, Molecular Diversity, Germplasm Accessions, Molecular Markers

Conflicts of Interest

The authors declare no conflicts of interest.

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