Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum

DOI: 10.4236/abb.2014.55051   PDF   HTML     4,858 Downloads   6,989 Views   Citations


In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.

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Shahzadi, T. , Anwar, Z. , Iqbal, Z. , Anjum, A. , Aqil, T. ,  , B. , Afzal, A. , Kamran, M. , Mehmood, S. and Irshad, M. (2014) Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum. Advances in Bioscience and Biotechnology, 5, 426-433. doi: 10.4236/abb.2014.55051.

Conflicts of Interest

The authors declare no conflicts of interest.


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