The Effects of Auxins and Cytokinin on Growth and Development of (Musa sp.) Var. “Yangambi” Explants in Tissue Culture


The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro. Explants were taken from young suckers of field grown plants of var. Yangambi. The shoot tips were cultured on MS media supplemented with different concentrations of BAP (0, 2, 4, 6 and 8 mg/l) with or without IAA at concentration of 0.34 mg/l. At the rooting phase, the media was supplemented with different concentrations of IBA (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) with or without BAP at concentration of 0.2 mg/l. The results indicated that 6.0 mg/l BAP significantly increased the number of shoots formed and the interaction of 6 mg/l BAP with 0.35 mg/l IAA significantly increased the fresh weight. For rooting, 2.0 mg/l IBA was more efficient in number and length of roots produced than all other treatments.

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M. Ngomuo, E. Mneney and P. Ndakidemi, "The Effects of Auxins and Cytokinin on Growth and Development of (Musa sp.) Var. “Yangambi” Explants in Tissue Culture," American Journal of Plant Sciences, Vol. 4 No. 11, 2013, pp. 2174-2180. doi: 10.4236/ajps.2013.411269.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ikram-ul-Haq and M. U. Dahot, “Morpho-Physiological Aspects of Micro-Propagating Banana under Different Hormonal Conditions,” Asian Journal of Plant Sciences, Vol. 6, No. 3, 2007, pp. 496-501.
[2] P. Madhulatha, M. Anbalagan, S. Jayachandran and N. Sakthivel, “Influence of Liquid Pulse Treatment with Growth Regulators on in Vitro Propagation of Banana (Musa spp. AAA),” Plant Cell Tissue Organ Culture, Vol. 76, No. 2, 2004, pp. 189-192.
[3] J. North, P. Ndakidemi and C. Laubscher, “Effects of Antioxidants, Plant Growth Regulators and Wounding on Phenolic Compound Excretion during Micropropagation of Strelitzia reginae,” International Journal of the Physical Sciences, Vol. 7, No. 4, 2012, pp. 638-646.
[4] R. A. Dixon and R. A. Gonzales, “Plant Cell Culture: A Practical Approach,” 2nd Edition, Oxford University Press, Oxford, 1994.
[5] N. Jafari, R. Y. Othman and N. Khalid, “Effect of Benzylaminopurine (BAP) Pulsing on in Vitro Shoot Multiplication of Musa acuminata (Banana) cv. Berangan,” African Journal of Biotechnology, Vol. 10, No. 13, 2011, pp. 2446-2450.
[6] C. M. Buising, R. C. Shoemaker and R. M. Benbow, “Early Events of Multiple Bud Formation and Shoot Development in Soybean Embryonic Axes Treated with the Cytokinin, 6-Benzylaminopurine,” American Journal of Botany, Vol. 81, No. 1, 1994, pp. 1435-1448.
[7] K. S. Alexandrova, P. D. Denchev and B. Y. Conger, “Micro Propagation of Switchgrass by Node Culture,” Crop Science, Vol. 36, No. 6, 1996, pp. 1709-1711.
[8] S. Bohidar, M. Thirunavoukkarasu and T. V. Roa, “Effect of Plant Growth Regulators on in Vitro Micro Propagation of ‘Garden Rue’ (R. graveolens L.),” International Journal of Integrated Biology, Vol. 3, No. 1, 2008, pp. 36-43.
[9] D. Vuylsteke, “Shoot-Tip Culture for the Propagation, Conservation and Exchange of Musa germplasm,” IBPGR, Rome, 1989.
[10] N. Hussein, “Effects of Nutrient Media Constituents on Growth and Development of Banana (Musa spp.) Shoot Tips Cultured in Vitro,” African Journal of Biotechnology, Vol. 11, No. 37, 2012, pp. 9001-9006.
[11] H. Gubbuk and M. Pekmezci, “In Vitro Propagation of Some New Banana Types (Musa spp.),” Turkish Journal of Agriculture and Forestry, Vol. 28, No. 5, 2004, pp. 355-361.
[12] D. Vuylsteke and E. Lanhe, “Feasibility of in Vitro Propagation of Bananas and Plantains,” Tropical Agriculture (Trinidad), Vol. 62, No. 20, 1985, pp. 323-328.
[13] S. Shirani, F. Mahdavi and M. Maziah, “Morphological Abnormality among Regenerated Shoots of Banana and Plantain (Musa spp.) after in Vitro Multiplication with TDZ and BAP from Excised Shoot Tips,” African Journal of Biotechnology, Vol. 8, No. 21, 2011, pp. 5755-5761.
[14] G. Arinaitwe, P. R. Rubaihayo and M. J. S. Magambo, “Effects of Auxin/Cytokinin Combinations on Shoot Proliferation in Banana Cultivars,” African Crop Science Journal, Vol. 7, No. 4, 1999, pp. 605-611.
[15] J. N. Buah, E. Danso, K. J. Taah, E. A. Abole, E. A. Bediako, J. Asiedu and R. Baidoo, “The Effects of Different Concentrations Cytokinins on the in Vitro Multiplication of Plantain (Musa sp.),” Biotechnology, Vol. 9, No. 3, 2010, pp. 343-347.
[16] J. North, P. Ndakidemi and C. P. Laubscher, “The Potential of Developing an in Vitro Method for Propagating Strelitziaceae,” African Journal Biotechnology, Vol. 9, No. 45, 2010, pp. 7583-7588.
[17] U. Bhosale, S. Dubhashi, N. Mali and H. Rathod, “In Vitro Shoot Multiplication in Different Species of Banana,” Asian Journal of Plant Science and Research, Vol. 1 No. 3, 2011, pp. 23-27.
[18] A. A. A. Sajid, N. H. Naveed, A. Majid, A. Saleem, A. U. Khan, F. I. Jafery and S. Naz, “Initiation, Proliferation and Development of Micro-Propagation System for Mass Scale Production of Banana through Meristem Culture,” African Journal of Biotechnology, Vol. 10, No. 70, 2011, pp. 15731-15738.
[19] A. Hagar, G. Debus, H. G. Edil, H. Strosky and R. Serrano, “Auxin Induces Exocytosis and the Rapid Synthesis of a High Turnover Pool of Plasma Membrane H+-APTase,” Planta, Vol. 185, No. 4, 1991, pp. 527-537.
[20] L. Venkatachalam, R. V. Sreedhar and N. Bhagyalakshmi, “Micro Propagation in Banana Using High Levels Does Not Involve Any Genetic Change as Revealed by RAPD and ISSR Markers,” Plant Growth Regulation, Vol. 51, No. 3, 2007, pp. 192-205.
[21] M. W. Bairu, W. A. Strik, K. Dolezal and J. V. Staden, “The Role of Topolins in Micro Propagation and Somaclonal Variation of Banana Cultivars ‘Williums’ and ‘Grand Naine’ (Musa spp. AAA),” Plant Cell, Tissue and Organ Culture, Vol. 95, No. 3, 2008, pp. 373-379.
[22] H. Strosse, I. Van den Houwe and B. Panis, “Banana Cell and Tissue Culture Review,” Science Publishers, Inc., Gainesville, 2004.
[23] G. Arinaitwe, P. R. Rubaihayo and M. J. S. Magambo, “Proliferation Rate Effects of Cytokinins on Banana (Musa spp.) Cultivars,” Scientia Horticulturae, Vol. 86, No. 1, 2000, pp. 13-21.
[24] D. Dhed’a, F. Dumortier, B. Panis, D. Vuylsteke and E. De Langhe, “Plant Regeneration in Cell Suspension Cultures of Cooking Banana ‘Bluggoe’ Cultivar (Musa spp. ABB Group),” Fruits, Vol. 46, No. 2, 1991, pp. 125-135.
[25] M. D. Al-Amin, M. R. Karim, M. R. Amin, S. Rahman and A. N. M Manun, “In Vitro Micro Propagation of Banana,” Bangladesh Journal of Agriculture Research, Vol. 34, No. 4, 2009, pp. 645-659.
[26] R. L. Jarret, W. Rodriguez and R. Fernandez, “Evaluation, Tissue Culture Propagation and Dissemination of ‘Saba’ and ‘Pelipita’ Plantains in Costa Rica,” Scientia Horticulturae, Vol. 25, No. 2, 1985, pp. 137-147.
[27] S. S. Cronauer and A. D. Krikorian, “Multiplication of Musa from Excised Stem Tips,” Annals of Botany, Vol. 53, No. 3, 1984, pp. 321-328.
[28] R. S. Raut and V. E. Lokhande, “Propagation of Plantain through Meristem Culture,” Annals of Plant Physiology, Vol. 3, No. 2, 1989, pp. 256-260.
[29] S. Rahman, N. Biswas, M. M. Hassan, M. G. Ahmed, A. Mamun, M. R. Islam, M. Moniruzzaman and M. E. Haque, “Micro Propagation of Banana (Musa sp.) cv. Agnishwar by in Vitro Shoot Tip Culture,” International Research Journal of Biotechnology, Vol. 4, No. 4, 2013, pp. 83-88.

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