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Assessment on early blight of potato in order to compare the two methods in vitro using pathogenic fungi Alternaria solani

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DOI: 10.4236/ns.2013.511145    4,017 Downloads   6,062 Views   Citations

ABSTRACT

Potato (Solanum tuberosum) early blight, caused by Alternaria solani is one of the most destructive fungal foliar diseases. This research was done in order to study methods comparison of evaluation by culture filtrate of A. solani in in vitro condition for selecting resistance cultivars to early blight. Plantlets of potato viruse free were obtained from the National plant gene bank of Iran, and were inoculated in vitro methods with a culture filtrate of A. solani. In in vitro selection by droplet of culture filtrate method, leaflet received a 10 μl droplet of the A. solani culture filtrate and in in vitro selection by direct using of culture filtrate method, plantlets were placed in test tubes that include 5 μl A. solani culture filtrate. The experimental design was factorial on basis of completely randomized design (CRD) with two factors, three replications and six genotypes. During droplet method assay, the A. solani symptoms appeared 1 - 2 days until 6 days and during direct method they appeared 2 - 3 days until 6 days. The AUDPC values were submitted to the analysis of varience (ANOVA) and AUDPC means were compared by using Duncan test (α = 0.01%). In each method, significant difference among potato cultivars was observed for disease to early blight (p < 0.01). Results show that casmos cultivar is susceptible for resistance to early blight and in vitro methods experiment had the same result.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Mirkarimi, H. , Abasi-Moghadam, A. and Mozafari, J. (2013) Assessment on early blight of potato in order to compare the two methods in vitro using pathogenic fungi Alternaria solani. Natural Science, 5, 1189-1192. doi: 10.4236/ns.2013.511145.

References

[1] Boiteux, L.S., Reifschneider, F.J.B., Fonseca, M.E. and Buso, J.A. (1995) Search for sources of early blight (Alternaria solani) field-resistance not associated with vegetative late maturity in tetraploid potato germplasm. Euphytica, 83, 63-70.
http://dx.doi.org/10.1007/BF01677862
[2] Nachmias, A., Caligari, P.D.S., Mackay, G.R. and Livescu, L. (1988) The effects of AIternaria. solani and Verticillium dahliae on potatoes growing in Israel. Potato Research, 31, 443-450.
http://dx.doi.org/10.1007/BF02357881
[3] Teng, P.S. and Bisonette, H.L. (1985) Potato yield losses due to early blight in Minnesota fields, 1981 and 1982. American Potato Journal, 62, 619-627.
http://dx.doi.org/10.1007/BF02854436
[4] Cassells, A.C. and Kowalski, B. (1998) Strategies for the evaluation of variation as a source of resistance to early and late blight of potato. In: Khurana, P., Chandra, R. and Mahesh, D., Eds., Comprehensive Potato Biotechnology, Malhotra Publishing House, New Delhi, 50-60.
[5] Christ, B.J. and Haynes, K.G.V. (2001) Inheritance to early blight disease in a diploid potato population. Plant Breeding, 120, 169-172.
http://dx.doi.org/10.1046/j.1439-0523.2001.00565.x
[6] Dita Rodriguez, M.A., Brommonschenkel, S.H., Matsuoka, K. and Mizubuti, E.S.G. (2006) Components of resistance to early blight in four potato cultivars: Effect of leaf position. Journal of Phytopathology, 154, 230-235.
http://dx.doi.org/10.1111/j.1439-0434.2006.01089.x
[7] Herriott, A.B., Haynes, J.F.L. and Shoemaker, P.B. (1990) Inheritance of resistance to early blight disease in tetraploid x diploid crosses of potatoes. Hortscience, 25, 224226.
[8] Lopes, C.A. and Reifschneider, F.J.B. (1999) Manejo integrado das doenc as da batata. Inf. Agropec, 20, 56-60.
[9] Martinez, P.R. and Sinclair, M. (1994) Selection in vitro of resistance to Early Blight (Alternaria solani Sorauer) in Creole Potatoes (Solanum phureja Junz). Fitopatol Colomb, 18, 90-100.
[10] Rodriguez, N.V., Kowalski, B., Rodriguez, L.G., Caraballoso, B., Suarez, M.A., Perez, P.O., Quintana, C.R., Gonzalez, N. and Ramos, R.Q. (2007) In vitro and ex vitro selection of potato plantlets for resistance to early blight. Journal of Phytopathology, 155, 582-586.
http://dx.doi.org/10.1111/j.1439-0434.2007.01282.x
[11] Shaner, G. and Finney, R.E. (1977) The effect of nitrogen fertilization in the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67, 1051-1056.
http://dx.doi.org/10.1094/Phyto-67-1051
[12] Pryor, B.M. and Michailides, T.J. (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology, 92, 406-416.
http://dx.doi.org/10.1094/PHYTO.2002.92.4.406
[13] Veitia, N., Dita, M.A., Garcia, L., Herrera, L., Bermu dez, I., Acosta, M., Clavero, J., Orellana, P., Romero, C. and Garcia, L. (2001) Use of tissue culture and the mutagenesis in vitro for the improvement of the resistance to Alternaria solani (Solanum tuberosum Lin.), to var Desiree. Biotecnol Veg, 1, 43-47.
[14] Locke, S.B. (1949) Resistance to early blight and Septoria leaf spot in the genus Lycopersicon. Phytopathology, 39, 829-836.

  
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