An Analysis of the Correlation between the Changes in Sa-tellite DNA Methylation Patterns and Plant Cell Responses to the Stress

DOI: 10.4236/cellbio.2013.23018   PDF   HTML     2,878 Downloads   5,050 Views   Citations


The differences in satellite DNA methylation pattern of corn seedlings with various spontaneous chromosome aberration yields and changes in methylation pattern of these DNA sequences under different exposure modes of acute UV-C and chronic gamma-irradiations have been investigated. The obtained experimental data and the conducted correlation analysis demonstrated the significant correlation between the satellite DNA methylation pattern varieties and chromosome aberration yields under various stress exposure modes. The role of satellite DNA methylation pattern variability and its changing in key responses to stress such as mobile elements’ activation, cell’s passage of checkpoints, and homological repair was discussed. 

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A. Sokolova, D. , S. Vengzhen, G. and P. Kravets, A. (2013) An Analysis of the Correlation between the Changes in Sa-tellite DNA Methylation Patterns and Plant Cell Responses to the Stress. CellBio, 2, 163-171. doi: 10.4236/cellbio.2013.23018.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] R. L. P. Adams, “DNA Methylation,” Principles of Medical Biology, Vol. 5, 1996, pp. 33-66. doi:10.1016/S1569-2582(96)80107-3
[2] E. N. Tishchenko and O. V. Dubrovnaya, “Epigenetic Regulation. DNA Methylation of Genes and Transgenes in Plants,” Logos, Kiev, 2004, 384 Pages.
[3] M.-T. Hauser, W. Aufsatz, C. Jonak and Ch. Luschnig, “Transgenerational Epigenetic Inheritance in Plants,” Biochimica at Biophysica Acta, Vol. 1809, No. 8, 2011, pp. 459-468. doi:10.1016/j.bbagrm.2011.03.007
[4] A. Agorio and P. Vera, “ARGONAUTE4 Is Required for Resistance to Pseudomonas syringae in Arabidopsis,” The Plant Cell, Vol. 19, No. 11, 2007, pp. 3778-3790. doi:10.1105/tpc.107.054494
[5] A. Bilichak, Y. Ilnystkyy, Y. Hollunder and I. Kovalchuk, “The Progeny of Arabidopsis Thaliana Plants Exposed to Salt Exhibit Changes in DNA Methylation, Histone Modifications and Gene Expression,” PLoS One, Vol. 7, No. 1, 2012, pp. 1-15.
[6] L. Zhong, Y. Xu and J. Wang, “DNA-Methylation Changes Induced by Salt Stress in Wheat Triticum aestivum,” African Journal of Biotechnology, Vol. 8, No. 22, 2009, pp. 6201-6207.
[7] O. Kovalchuk, P. Burke, A. Arkhipov, N. Kuchma, S. J. James, I. Kovalchuk and I. Pogribny, “Genome Hyper- methylation in Pinus silvestris of Chernobyl—A Mecha- nism for Radiation Adaptation?” Mutation Research, Vol. 529, No. 1-2, 2003, pp. 13-20. doi:10.1016/S0027-5107(03)00103-9
[8] I. Kovalchuk, V. Abramov, I. Pogribny and O. Kovalchuk, “Molecular Aspects of Plant Adaptation to Life in the Chernobyl Zone,” Plant Physiology, Vol. 135, No. 1, 2004, pp. 357-363. doi:10.1104/pp.104.040477
[9] I. Pogribny, I. Koturbash, V. Tryndyak, D. Hudson, S. M. L. Stevenson, O. Sedelnikova, W. Bonner and O. Kovalchuk, “Fractionated Low-Dose Radiation Exposure Leads to Accumulation of DNA Damage and Profound Alterations in DNA and Histone Methylation in the Murine Thymus,” Molecular Cancer Research, Vol. 3, No. 10, 2005, pp. 553-561. doi:10.1158/1541-7786.MCR-05-0074
[10] A. P. Kravets, T. A. Mousseau, A. V. Litvinchuk, Sh. Ostermiller and G. Vengzhen, “Changes in DNA Methylation Pattern in Weat Plants under Chronical γ—Exposure of Seeds,” Cytology and Genetics, Vol. 44, No. 5, 2010, pp. 18-22. doi:10.3103/S0095452710050038
[11] M. A. Coleman, E. Yin and L. Peterson, “Low-Dose Irradiation Alters the Transcript Profiles of Human Lymphoblastoid Cells Including Genes Associated with Cytogenetic Radioadaptive Response,” Radiation Research, Vol. 164, No. 4, 2005, pp. 369-382. doi:10.1667/RR3356.1
[12] M. Banda, A. Bommineni, R. A. Thomas, L. S. Luckinbill and J. D. Tucker, “Evaluation and Validation of Housekeeping Genes in Response to Ionizing Radiation and Chemical Exposure for Normalizing RNA Expression in Real-Time PCR,” Mutation Research, Vol. 8, No. 649, 2008, pp. 126-134.
[13] A. M. Serebryanyi, “Radiation Adaptive Response as a Stress Reaction of a Cell,” Radiatsionnaya Biologiya. Radioekologiya, Vol. 51, No. 4, 2011, pp. 399-405.
[14] A. Kravets, D. Sokolova, G. Vengzhen and D. Grodzinsky “Corn Plant DNA Methylation Pattern Changes at UV-C Irradiation Fractionating,” Cytology and Genetics, Vol. 47, No. 1, 2013, pp. 29-35. doi:10.3103/S0095452713010052
[15] V. Hemleben, T. G. Beridze, L. Bakhman, Y. Kovarik and R. Torres, “Satellite DNA,” Uspehi Biologicheskoy Khimii, Vol. 43, 2003, pp. 267-306.
[16] F. M. Ausubel, “Current Protocols in Molecular Biology,” Biophotometer Operating Manual, 2004.
[17] J. M. S. Bartlett and D. Stirling, “PCR Protocols,” Humana Press Incorporate, Humana, 2003.
[18] Y. M. Tikunov and L. I. Khrystaleva, “Application of ISSR Markers in the Genus Lycopersicon,” Euphitica, Vol. 131, No. 1, 2003, pp. 71-80. doi:10.1023/A:1023090318492
[19] A. V. Lakin, “Biometry,” High School, Moskva, 1990.
[20] A. I. Gaziev, “Deterioration of Critical DNA Damage Repair Efficiency under Low Dose Irradiation,” Radiatsionnaya Biologiya Radioekologiya, Vol. 51, No. 5, 2011, pp. 512-529.

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