Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)

DOI: 10.4236/ajmb.2013.33020   PDF   HTML     4,342 Downloads   9,244 Views   Citations

Abstract

One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has golden yellow fruit flesh without smell. Crossbreeding of those two cultivars, in order to generate superior Durian cultivars has been done. Genetic diversity of durian generates from cross breeding between D. kutejensis and D. zibethinus was identified in molecular level using RAPD technique. Among 20 primers used in this study, 5 primers: OPA-02, OPA-03, OPA-08, OPA-10 and OPA-13 were capable of differentiating both the parents and the hybrids. RAPD analysis resulted in genetic diversity of hybrid Durian with family relationship of 0.59%-0.1%. Hybrids UB1, UB5, UB13, UB19, UB21, UB7 and UB35 have similarity value of 0.81% with their parent DRCK, whereas hybrids UB8, UB10, UB18 and UB17 have similarity value of 0.70% with their parent DRCM1. Hybrids UB2, UB16 and UB22 belong to one group with similarity value of 0.67%. Three hybrids lines UB2, UB16 and UB22 show the highest distance to both parent. The rest of the hybrids lines grouped into similar cluster to the parents D. kutejensis, whereas the other parent (D. zibethinus) belong to different cluster separated from all other hybrid lines and parents.

Share and Cite:

Hariyati, T. , Kusnadi, J. and Arumingtyas, E. (2013) Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs). American Journal of Molecular Biology, 3, 153-157. doi: 10.4236/ajmb.2013.33020.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Sastrapradja, S.D. and Rifai, M. (1989) Mengenal sumber pangan nabati dan suber plasma nutfahnya (knowing the vegetable and the germ plasm sources). Germplasm Preservation Commission and the National Center for Biotechnology, Indonesian Institute of Sciences, Bogor.
[2] Uji, T. (2005) Keanekaragaman jenis dan sumber plasma nutfah Durio (Durio spp.) di Indonesia. Herbarium Bogoriense, Bidang Botani, Pusat Penelitian Biologi-LIPI. Bulletin Plasma Nutfah, 11, 28-33.
[3] Subhadrabandhu, S., Schneemann, J.M.P. and Verheij, E.W.M. (1991) Edible Fruits and Nuts. Plant Resources of South-East Asia (PROSEA). Pudoc Wageningen, The Netherland.
[4] Bansir, L. Ashasri, S. and Awaluddin, M.S. (2010) Crossing of Durian between species (Durio zibethinus X Durio kutejensis). Simposium National Horticultura, Bali, 25-26 November 2010.
[5] Gewin, V. (2003) Genetically modified corn. Enviromental benefits and risks. PLoS Biology, 1, e8. doi:10.1371/journal.pbio.0000008
[6] Costa e Silva, J., Potts, B.M. and Dutkowski, G.W. (2006) Genotype by environment interaction for growth of Eucalyptus globulus in Australia. Tree Genetics and Genomes, 2, 61-75. doi:10.1007/s11295-005-0025-x
[7] Trujillo, I., Raloo, L. and Arus, P. (1995) Identifying olive cultivars by isozime analysis. Journal of the American Society for Horticultural Science, 120, 318-324.
[8] Weising, K., Nybom, H., Wolff, K. and Meyer, W. (2005) DNA fingerprinting in plant. Principles, Methods and Applications. 2nd Edition, CRC Press, Boca Raton. doi:10.1201/9781420040043
[9] Baig, M.N.R., Sapna, G. and Santhosh, D. (2009) Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers. Turkish Journal of Agriculture and Forestry, 33, 375-384.
[10] Nair, N.V, Selvi, A., Sreenivasan, T.V and Pushphalatha, K.N. (2002) Molecular diversity in Indian sugarcane cultivars as revealed by random amplified DNA polymerphisms. Euphytica, 127, 219-225. doi:10.1023/A:1020234428681
[11] Ruwaidah, I.P., Supriyadi and Parjanto. (2009) Variability analysis of sukun durian plant (Durio zibethinus) based on RAPD marker. Nusantara Biociense, 1, 84-91.
[12] Karatas, H. and Agaoglu, Y.S. (2010) RAPD analysis of selected local Turkish grape cultivars (Vitis vinifera). Genetics and Molecular Research, 9, 1980-1986. doi:10.4238/vol9-4gmr926
[13] Rocha, E.A., Luciano V.P., de Carvalho, H.H. and Claudia, T.G. ( 2009) Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. Crop Breeding and Applied Biotechnology, 10, 204-210. doi:10.1590/S1984-70332010000300004
[14] Arumingtyas, E.L., Widoretno, W. and Indriyani, S. (2012) Somaclonal variations of Soybeans (Glycine Max. L. Merr) stimulated by drought stress based on random amplified polymorphic DNAs (RAPDs). American Journal of Molecular Biology, 2, 85-91. doi:10.4236/ajmb.2012.21009
[15] Arumingtyas, E.L., Munawarti, A., Indriyani, S. and Sudjindro (2010) Polymorphism analysis of kenaf (Hibiscus cannabinus L.) mutants based on random amplified polymorphic DNAs (RAPDs). Journal of Materials Science and Engineering, 4, 56-62.
[16] Patamsyte, J., Tatjana, C., Donatas, N., Violeta, K., Virginija, V., Vytautas, R. and Donatas, Z. (2011) Genetic diversity of warty cabbage (Bunias orientalis L.) revealed by RAPD ans ISSR markers. Zemdirbyste Agricultur, 98, 293-300.
[17] Chaudhary, L., Sindhu, A., Kumar M., Kumar R. and Saini, M. (2010). Estimation of genetic divergence among some cotton varieties by RAPD analysis. Journal of Plant Breeding and Crop Science, 2, 39-43.
[18] Syafaruddin and Santoso, T.J. (2011) Optimasi teknik isolasi dan purifikasi dna yang efisien dan efektif pada kemiri Sunan (Reutalis trisperma (Blanco) Airy Shaw) (Optimation of DNA isolation and purification techniques on Reutalis trisperma (Blanco) Airy Shaw). Jurnal Litri, 17, 11-17.
[19] Hutami, S., Mariska, I. and Supriati, Y. (2006) Improved plant genetic diversity through somaclonal diversity. Journal AgroBiogen, 2, 81-88.
[20] Martono, B. (2009) Genetic variability, heritability and correlation between quantitative characters Patchouli (Pogostemon sp.) Protoplasts fusion results. Jurnal Litri, 15, 9-14.
[21] Popov, V.N., Urbanovich, O.Y. and Kirichenko, V.V. (2002) Studying genetic diversity in inbred sunflower lines by RAPD and isozyme analyses. Russian Journal of Genetics, 38, 785-790. doi:10.1023/A:1016391621756
[22] Acquaah, G. (2007) Principles of plant genetics and breeding. 2nd Edition, Wiley-Blackwell, Hoboken.
[23] Mondini, L., Noorani, A. and Pagnotta, M.A. (2009) Assessing plant genetic diversity by molecular tools. Diversity, 1, 19-35.
[24] Hartl, D.L. and Jones, E.W. (2005) Essential genetics: A genomics perspective. 4th Edition, Jones & Bartlett Publishers, Burlington.
[25] Samal, S., Rout, G.R. and Lenka, P.C. (2003) Analysis of genetic relationship between population of cashew (Anacardium occudentale L.) by using RAPD markers morpological and characterization. Plant Soil Environment, 49, 176-182.

  
comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.