Mass production, fermentation, formulation of Pseudomonas putida for controlling of die back and phomopsis diseases on grapevine


The export oriented agricultural and horticultural crops depends on the export of residue free produce and has created a great potential and demand for the incorporation of biopesticides in crop protection. PGPR with wide scope for commercialization includes Pseudomonas putida. Pseudomonas putida was effective in reducing die back caused by a fungus Botryodiplodia theobromae and phomopsis or arm death, caused by a fungus, Phomopsis viticola of grapevine in vitro and in vivo. Pseudomonas putida showed optimum Siderophore pseudobactin production at 72 h, and growth peak at 120 h. glycerol as a source of carbon and yeast as a source of nitrogen Pseudomonas putida was formulated using polysaccharide dextran starch, which led to the elongation of conservation and the effectiveness of the biofungicide. Pseudomonas putida was very effective as biocontrol agents to reduce the die back and phomopsis diseases on grapevine. The obtained results showed that both bacterial treatments significantly increased the growth parameters as well as fruits dry weights and yield of grapevine.

Share and Cite:

Haggag, W. , Saleh, M. , Mostafa, I. and Adel, N. (2013) Mass production, fermentation, formulation of Pseudomonas putida for controlling of die back and phomopsis diseases on grapevine. Advances in Bioscience and Biotechnology, 4, 741-750. doi: 10.4236/abb.2013.46098.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Pearson, R. and Goheen, A. (1998) Compendium of grape diseases.
[2] Van Niekerk, J.M., Fourie, P., Halleen, F. and Crous, P.W. (2006) Botryosphaeria spp. as grapevine trunk disease pathogens. Phytopathologia Mediterranea, 45, S43S54.
[3] Ùrbez-Torres, J.R., Leavitt, G.M., Guerrero, J.C., Guevara, J. and Gubler, W.D. (2008). Identification and pathogenicity of Lasiodiplodia theobromae and Diplodiaseriata, the causal agents of bot canker disease of grapevines in Mexico. Plant Disease, 92, 519-529. doi:10.1094/PDIS-92-4-0519
[4] Trapman, M., Maxin, P. and Weber, R.W.S. (2007) Diplodiaseriata, cause of black fruit rot in organically grown apples in Holland, Belgium and Northern Germany.
[5] Kaliterna, J., Milicevic, T. and Cvjetkovic, B. (2009). Nove-spoznaje o etiologijicrnepjegavostivinoveloze i taksonomijivrstapovezanih s patogenom Phomopsis viticola (Sacc.) Sacc. [New Insight into Aetiology of Phomopsis Cane and Leaf Spot of Grapevine and Taxonomy of Species Related to Phomopsisviticola (Sacc.) Sacc., in Croatian]. Glasilobiljne Zastite, 5, 291-295.
[6] Dhanushka, U., Xingzhong, L., McKenzie, E.H.C., Chukeatirote, E., Bahkali, A.H.A. and Hyde, K.D. (2011). The genus Phomopsis: Biology, applications, species concepts and names of common phytopathogens. Fungal Diversity, 50, 189-225. doi:10.1007/s13225-011-0126-9
[7] Rombough, L. (2002) The grape grower, a guide to organic viticulture. Chelsea Green Publishing, 91-92.
[8] Haggag, W.M. (2002) Sustainable agriculture management of plant diseases. Journal of Biological Science, 2, 280284.
[9] Haggag, W.M. (2003) Biological control of plant diseases, and its developments. Prospects of the recent agricultural research. The Second Scientific Conference, National Research Center, 45.
[10] Wilson, M. (1997) Biocontrol of aerial plant diseases in agriculture and horticulture: Current approaches and future prospects. Journal of Industrial Microbiology and Biotechnology, 19, 188-191. doi:10.1038/sj.jim.2900436
[11] Meziane, H., Vander Sluis, I., VanLoon, L.C., Hofte, M. and Bakker, P.A.H.M. (2005) Determinants of Pseudomonas putida WCS358 involved in inducing systemic resistance in plants. Molecular Plant Pathology, 6, 177185. doi:10.1111/j.1364-3703.2005.00276.x
[12] Leeman, M., DenOuden, E.M., VanPelt, J.A., Dirkx, F.P.M., Steijl, H., Bakker, P.A.H.M. and Schippers, B. (1996) Iron availability affects induction of systemic resistance to fusarium wilt of radish by Pseudomonas fluorescens. Phytopathology, 86, 149-155. doi:10.1094/Phyto-86-149
[13] Leeman, M., VanPelt, J.A., DenOuden, F.M., Heinsbroek, M., Bakker, P.A.H.M. and Schippers, B. (1995) Induction of systemic resistance against fusarium wilt of radish by lipopolysaccharides of Pseudomonas fluorescens. Phytopathology, 85, 1021-1027. doi:10.1094/Phyto-85-1021
[14] Vidhyasekaran, P. and Muthamilan, M. (1995) Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Disease, 79, 782-786. doi:10.1094/PD-79-0782
[15] Duffy, B.K. and Defago, G. (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied and Environmental Microbiology, 65, 2429-2438.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.