Sealing ability of AH26 and soft core gutta-percha using different instrumentations techniques and irrigations protocol: A SEM evaluation

Abstract

Aim: The aim of this ex vivo study, with SEM, was to determine the effects of the smear layer on the root canal systems after different instrumentation technique and irrigation protocol, obturated with carrier based gutta-percha (Soft Core) and AH26 sealer. Materials & Methods: Forty freshly extracted human teeth were used for this study. The teeth were divided into two major groups according to instrumentation technique (Manual and ProFile) and then each major group into four subgroups according to irrigation protocol (Saline solution, 17% EDTA, 5.25% NaOCl). All of the samples were obturated with AH26 and Soft Core Heating Gutta-percha. The evaluation of the objectives was performed using a Scanning Electron Microscope. Result: ProFile instrumentation followed with irrigation of 17% EDTA and 5.25% NaOCl, selaed with AH26 Soft Core Heating Guttapercha provided maximal contact at the S/D interface. The S/D interface had a serrated appearance due to effective penetration of the sealer into the patent dentin tubules. Conclusions: The adhezion of the respective sealer is more successful in cases when smear layer was removed completely.

Share and Cite:

Koçani, F. , Kamberi, B. and Dragusha, E. (2013) Sealing ability of AH26 and soft core gutta-percha using different instrumentations techniques and irrigations protocol: A SEM evaluation. Open Journal of Stomatology, 3, 163-170. doi: 10.4236/ojst.2013.32029.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Schilder, H. (1967) Filling root canals in three dimensions. Dental Clinics of North America, 11, 723-744.
[2] Sjögren, U., Hägglund, B., Sundqvist, G. and Wing, K. (1990) Factors affecting the long-term results of endodontic treatment. Journal of Endodontics, 16, 498-504. doi:10.1016/S0099-2399(07)80180-4
[3] Buckley, M. and Spаngberg, W. (1995) The prevalence and technical quality of endodontic treatment in an American subpopulation. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 79, 92100.
[4] Sundqvist, G., Figdor, D., Persson, S. and Sjögren, U. (1998) Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 85, 86-93. doi:10.1016/S1079-2104(98)90404-8
[5] Katebzadeh, N., Hupp, J. and Trope, M. (1999) Histological periapical repair after obturation of infected root canals in dogs. Journal of Endodontics, 25, 364-368. doi:10.1016/S0099-2399(06)81173-8
[6] Leonard, J.E., Gutmann, J.L. and Guo, I.Y. (1996) Apical and coronal seal of roots obturated with a dentine bonding agent and resin. International Endodontic Journal, 29, 76-83. doi:10.1111/j.1365-2591.1996.tb01165.x
[7] Mannocci, F. and Ferrari, M. (1998) Apical seal of roots obturated with laterally condensed gutta-percha, epoxy resin cement, and dentin bonding agent. Journal of Endodontics, 24, 41-44. doi:10.1016/S0099-2399(98)80212-4
[8] Ricucci, D. and Langeland, K. (1998) Apical limit of root canal instrumentation and obturation, part 2. Histological study. International Endodontic Journal, 31, 394-409. doi:10.1046/j.1365-2591.1998.00183.x
[9] Pommel, L., Jacquot, B. and Camps, J. (2001) Lack of Correlation among three methods for evaluation of apical leakage. Journal of Endodontics, 27, 347-350. doi:10.1097/00004770-200105000-00010
[10] Cobankara, F.K., Orucoglu, H., Sengun, A. and Belli, S. (2006) The quantitative evaluation of apical sealing of four endodontic sealers. Journal of Endodontics, 32, 6668. doi:10.1016/j.joen.2005.10.019
[11] Frank, J.V. (1984) Root canal anatomy of the human permanent teeth. Oral Surgery, Oral Medicine, Oral Pathology, 58, 589-599. doi:10.1016/0030-4220(84)90085-9
[12] Çaliskan, M.K., Pehlivan, Y., Sepetçioglu, F., Türkün, M. and Tuncer, S.S. (1995) Root canal morphology of human permanent teeth in a Turkish population. Journal of Endodontics, 21, 200-204. doi:10.1016/S0099-2399(06)80566-2
[13] White, R.R., Goldman, M. and Lin, P.S. (1984) The influence of the smeared layer upon dentinal tubule penetration by plastic filling materials. Journal of Endodontics, 10, 558-562. doi:10.1016/S0099-2399(84)80100-4
[14] Koçani, F., Kamberi, B., Dragusha, E., Mrasori, S. and Haliti, F. (2012) The cleaning efficiency of the root canal after different instrumentation technique and irrigation protocol: A SEM analysis. Open Journal of Stomatology, 2, 69-76.
[15] Koçani, F., Kamberi, B. and Dragusha, E. (2012) Manual sonic-air and ultrasonic instrumentation of root canal and irrigation with 5.25% sodium hypochlorite and 17% Ethylenediaminetetraacetic acid: A scanning electron microscope study. Journal of Conservative Dentistry, 15, 118-122. doi:10.4103/0972-0707.94575
[16] Moorer, W.R. and Wesselink, P.R. (1982) Factors promoting the tissue dissolving capability of sodium hypochlorite. International Endodontic Journal, 15, 187-196. doi:10.1111/j.1365-2591.1982.tb01277.x
[17] Sudha, R., Sukumaran, V.R., Ranganathan, J. and Bharadwaj, N. (2006) Comparative evaluation of the effect of two different concentrations of EDTA at two different PH and time periods on root dentin. Journal of Conservative Dentistry, 9, 36-42. doi:10.4103/0972-0707.41307
[18] Torabinejad, M., Khademi, A.A., Babagoli, J., Cho, Y., Johnson, W.B., Bozhilov, K., et al. (2003) A new solution for the removal of smear layer. Journal of Endodontology, 29, 170-175. doi:10.1097/00004770-200303000-00002
[19] Ballal, N.V., Kandian, S., Mala, K., Bhat, K.S. and Acharya, S. (2009) Comparison of the efficacy of maleic acid and ethylenediaminetetraacetic acid in smear layer removal from instrumented human root canal: A scanning electron microscopic study. Journal of Endodontology, 35, 1573-1576. doi:10.1016/j.joen.2009.07.021
[20] Rai, B., Jain, R., Kharb, S., Miglani, S. and Anand, S.C. (2006) Comparative anti-microbial activity of 15% EDTA and 15% dimercaptosuccinic acid (DMSA). Journal of Conservative Dentistry, 9, 32-35. doi:10.4103/0972-0707.41306
[21] Goldberg, F., Bernat, M.I., Spielberg, C., Massone, E.J. and Piovano, S.A. (1985) Analysis of the effect of ethylenediaminetetraacetic acid on the apical seal of root canal fillings. Journal of Endodontics, 11, 544-547. doi:10.1016/S0099-2399(85)80200-4
[22] Lares, C. and ElDeeb, M.E. (1990) The sealing ability of the thermafil obturation technique. Journal of endodontics, 16, 474-479. doi:10.1016/S0099-2399(07)80176-2
[23] Gençoglu, N., Samani, S. and Günday, M. (1993) Dentinal wall adaptation of thermoplasticized gutta-percha in the absence or presence of smear layer: A scanning electron microscopic study. Journal of Endodontics, 19, 558- 562. doi:10.1016/S0099-2399(06)81286-0
[24] Lee, K.-W., Williams, M.C., Camps, J.J. and Pashley, D.H. (2002) Adhesion of endodontic sealers to dentin and gutta-percha. Journal of Endodontics, 28, 684-688. doi:10.1097/00004770-200210000-00002
[25] Zielinski, T.M., Baumgartner, J.C. and Marshall, J.G. (2008) An evaluation of gutta flow and gutta-percha in the filling of lateral grooves and depressions. Journal of Endodontics, 34, 295-298. doi:10.1016/j.joen.2007.12.004
[26] Saleh, I.M., Ruyter, I.E., Haapasalo, M.P. and Ørstavik, D. (2003) Adhesion of endodontic sealers: Scanning electron microscopy and energy dispersive spectroscopy. Journal of Endodontics, 29, 595-601. doi:10.1097/00004770-200309000-00013
[27] Limkangwalmongkol, S., Burtscher, P., Abbott, P.V., Sandler, A.B. and Bishop, B.M. (1991) A comparative study of the apical leakage of four root canal sealers and laterally condensed gutta-percha. Journal of Endodontics, 17, 495-499. doi:10.1016/S0099-2399(06)81797-8
[28] Oguntebi, B.R. and Shen, C. (1992) Effect of different sealers on thermoplasticized gutta-percha root canal obturations. Journal of Endodontics, 18, 363-366. doi:10.1016/S0099-2399(06)81219-7
[29] Wu, M.-K., De Gee, A.J. and Wesselink, P.R. (1997) Leakage of AH26 and ketac-endo used with injected warm gutta-percha. Journal of Endodontics, 23, 331-334. doi:10.1016/S0099-2399(97)80417-7
[30] Nagas, E., Altundasar, E. and Serper, A. (2009) The effect of master point taper on bond strength and apical sealing ability of different root canal sealers. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 107, e61-e4. doi:10.1016/j.tripleo.2008.09.020
[31] Mc Comb, D. and Smith, C. (1975) A preliminary scanning electron microscopy study of root after endodontic procedures. Journal of Endodontics, 1, 238-242. doi:10.1016/S0099-2399(75)80226-3
[32] Lester, K.S. and Boyde, A. (1977) Scaning electron microscopy of instrumented, irigated and filled root canals. British Dental Journal, 143, 359-367. doi:10.1038/sj.bdj.4804007
[33] Goldman, M., Goldman, L.B., Cavaleri, R., Bogis, J. and Sun, L.P. (1982) The efficacy of several endodontic irrigating solutions: A scanning electron microscopic study: Part 2. Journal of endodontics, 8, 487-492. doi:10.1016/S0099-2399(82)80073-3
[34] Evans, J.T. and Simon, J.H.S. (1986) Evaluation of the apical seal produced by injected thermoplasticized guttapercha in the absence of smear layer and root canal sealer. Journal of Endodontics, 12, 101-107. doi:10.1016/S0099-2399(86)80299-0
[35] Goldman, M., Goldman, L.B., Cavaleri, R., Bogis, J. and Lin, P.S. (1982) The efficacy of several endodontic irrigating solutions: A scanning electron microscopic study: Part 2. Journal of National Cancer Institute, 8, 487-492. doi:10.1016/S0099-2399(82)80073-3
[36] Gettleman, B.H., Messer, H.H. and ElDeeb, M.E. (1991) Adhesion of sealer cements to dentin with and without the smear layer. Journal of Endodontics, 17, 15-20. doi:10.1016/S0099-2399(07)80155-5
[37] Georgopoulou, M.K., Wu, M.-K., Nikolaou, A. and Wesselink, P.R. (1995) Effect of thickness on the sealing ability of some root canal sealers. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 80, 338-344. doi:10.1016/S1079-2104(05)80392-0
[38] Kokkas, A.B, Boutsioukis, A.C., Vassiliadis, L.P. and Stavrianos, C.K. (2004) The influence of the smear layer on dentinal tubule penetration depth by three different root canal sealers: An in vitro study. Journal of Endodontics, 30, 100-102. doi:10.1097/00004770-200402000-00009
[39] Ordinola-Zapata, R., Bramante, C.M., Graeff, M.S.Z., et al. (2009) Depth and percentage of penetration of endodontic sealers into dentinal tubules after root canal obturation using a lateral compaction technique: A confocal laser scanning microscopy study. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 108, 450-457. doi:10.1016/j.tripleo.2009.04.024
[40] Oruçoglu, H., Sengun, A. and Yilmaz, N. (2005) Apical leakage of resin based root canal sealers with a new computerized fluid filtration meter. Journal of Endodontics, 31, 886-890. doi:10.1097/01.don.0000164134.79052.b3
[41] Özok, A.R., Van Der Sluis, L.W.M., Wu, M.-K. and Wesselink, P.R. (2008) Sealing ability of a new polydimethylsiloxane-based root canal filling material. Journal of Endodontics, 34, 204-207. doi:10.1016/S0099-2399(87)80195-4
[42] White, R.R., Goldman, M. and Lin, P.S. (1987) The influence of the smeared layer upon dentinal tubule penetration by endodontic filling materials. Part II. Journal of Endodontics, 13, 369-374. doi:10.1046/j.1365-2842.2003.01084.x
[43] Sevimay, S. and Dalat, D. (2002) Evaluation of penetration and adaptation of three different sealers: A SEM study. Journal of Oral Rehabilitation, 30, 951-955. doi:10.1046/j.1365-2842.2003.01084.x
[44] Suprabha, B., Sudha, P. and Vidya, M. (2002) A comparative evaluation of sealing ability of root canal sealers. Indian Journal of Dental Research, 13, 31-36.
[45] Sousa-Neto, M., Passarinho-Neto, J., Carvalho-Junior, J., Cruz-Filho, A., Pecora, J. and Saquy P. (2002) Evaluation of the effect of EDTA, EGTA and CDTA on dentin adhesiveness and microleakage with different root canal sealers. Brazilian Dental Journal, 13, 123-128. doi:10.1590/S0103-64402002000200009
[46] Schäfer, E. and Zapke, K. (2000) A comparative scanning electron microscopic investigation of the efficacy of manual and automated instrumentation of root canals. Journal of Endodontics, 26, 660-664. doi:10.1097/00004770-200011000-00007
[47] Hülsmann, M., Schade, M. and Schäfers, F. (2001) A comparative study of root canal preparation with HERO 642 and Quantec SC rotary Ni-Ti instruments. International Endodontic Journal, 34, 538-546. doi:10.1046/j.1365-2591.2001.00431.x
[48] Versümer, J., Hülsmann, M. and Schäfers, F. (2002) A comparative study of root canal preparation using ProFile.04 and Lightspeed rotary Ni-Ti instruments. International Endodontic Journal, 35, 37-46. doi:10.1046/j.1365-2591.2002.00454.x
[49] Mayer, B.E., Peters, O.A. and Barbakow, F. (2002) Effects of rotary instruments and ultrasonic irrigation on debris and smear layer scores: a scanning electron microscopic study. International Endodontic Journal, 35, 582589. doi:10.1046/j.1365-2591.2002.00502.x
[50] McComb, D. and Smith, C. (1975) A preliminary scanning electron microscopy study of root after endodontic procedures. Journal of Endodontics, 1, 238-242. doi:10.1016/S0099-2399(75)80226-3
[51] Goldman, L., Goldman, M. and Kronman, J. (1981) The efficacy of several irrigating solutions for endodontics: A scanning electron microscopic study. Oral Surgery, 52, 197-204. doi:10.1016/0030-4220(81)90319-4
[52] Mader, C., Baumgartner, J. and Peters, D. (1984) Scanning electron microscopic investigation of the smeared layer on root canal walls. Journal of Endodontics, 10, 477-483. doi:10.1016/S0099-2399(84)80204-6
[53] Thompson, S. and Dummer, P. (1997) Shaping ability of ProFile.04 Taper Series 29 rotary nickel-titanium instruments in simulated root canals. International Endodontic Journal, 30, 8-15.
[54] Bertrand, M., Pizzardini, P., Muller, M., Médioni, E. and Rocca, J. (1999) The removal of the smear layer using the Quantec system. A study using scanning electron microscope. International Endodontic Journal, 32, 217-224. doi:10.1046/j.1365-2591.1999.00231.x
[55] Gambarini, G. (1999) Shaping and cleaning the root canal system: A scanning electron microscopic evaluation of a new instrumentation and irrigation technique. Journal of Endodontics, 25, 800-803. doi:10.1016/S0099-2399(99)80300-8
[56] Gambarini, G. and Laszkiewicz, J.A. (2002) scanning electron microscopic study of debris and smear layer remaining following use of GT rotary instruments. International Endodontic Journal, 35, 422-427. doi:10.1046/j.1365-2591.2002.00495.x
[57] Siqueira, J.J., Araújo, P., Garcia, P., Fraga, R. and Sabóia Dantas, C. (1997) Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals. Journal of Endodontics, 23, 499-502. doi:10.1016/S0099-2399(97)80309-3
[58] Prati C, Foschi F, Nucci C, Montebugnoli L and Marcionni S. (2004) Appearance of the root canal walls after preparation with NiTi rotary instruments: A comparative SEM investigation. Clinical Oral Investigations, 8, 102-110. doi:10.1007/s00784-004-0253-8
[59] Ahlquist, M., Henningsonn, O., Hultenby, K. and Ohlin, J. (2001) The effectiveness of manual and rotary technique in the cleaning of root canals: A scanning electron microscopy study. International Endodontic Journal, 34, 533-537. doi:10.1046/j.1365-2591.2001.00429.x
[60] Foschi, F., Nucci, C., Montebugnoli, L., Marchionni, S., Breschi, L. and Malagnino, V. (2004) SEM evaluation of canal wall dentine following use of Mtwo and ProTaper NiTi rotary instruments. International Endodontic Journal, 37, 832-829. doi:10.1111/j.1365-2591.2004.00887.x
[61] Schafer, E. and Schlingemann, R. (2003) Efficiency of rotary nickel-titanium FlexMaster instruments compared with stainless steel hand K-Flexofile. Part 2. Cleaning effectiveness and instrumentation result in severely curved root canals of extracted teeth. International Endodontic Journal, 35, 514-521. doi:10.1046/j.1365-2591.2002.00515.x
[62] Guelzow, A., Stamm, O., Martus, P. and Kielbassa, A. (2005) Comparative study of six rotary nickel-titanium systems and hand instrumentation for root canal preparation International Endodontic Journal, 38, 743-752. doi:10.1111/j.1365-2591.2005.01010.x
[63] Yoshimine, Y., Ono, M. and Akamine, A. (2005) The shaping effects of three nickel-titanium rotary instruments in simulated S-shaped canals. Journal of Endodontology, 31, 373-375. doi:10.1097/01.don.0000140568.40462.43
[64] Iqbal, M., Firic, S., Tulcan, J., karabucak, B. and Kim, S. (2004) Comparision of apical transportation between ProFile and ProTaper NiTi rotary instruments. International Endodontic Journal, 37, 359-364. doi:10.1111/j.1365-2591.2004.00792.x
[65] Peters, O., Peters, C., Schonenberger, K. and Barbakow, F. (2003) ProTaper rotary root canal preparation: Effect of canal anatomy on final shape analyzed by micro CT. International Endodontic Journal, 36, 86-92. doi:10.1046/j.1365-2591.2003.00626.x
[66] Baumgartner, J. and Mader, C. (1987) A scanning electron microscopic evaluation of four root canal irrigation regimens. Journal of Endodontics, 13, 147-157. doi:10.1016/S0099-2399(87)80132-2

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.