Photokilling of Escherichia coli Using Hybrid Titania Nanoparticles Suspended in an Aqueous Liquid

Christophe Massard, Muriel Bonnet, Philippe Veisseire, Yves Sibaud, Eric Caudron, Komla Oscar Awitor

Clermont Université, Université d’Auvergne, C-BIOSENSS EA-4676 Clermont-Ferrand, France.
Clermont Université, Université d’Auvergne, Laboratoire de Biologie, IUT Génie Biologique, Aurillac, France.

DOI: 10.4236/jbnb.2013.42019                

Abstract

In this work, the photokilling of Escherichia coli using a “one-pot” synthesized suspension of anatase crystallized nanoparticles is evaluated. Preliminary to the biological tests concerning the antibacterial efficiency, the fabricated suspension, using a derived solgel process in soft chemistry condition, is characterized. Structural properties of the nanoparticles are investigated using Electronic Transmission Microscopy (TEM) equipped with Selected Area Electron Diffraction (SAED) probe and X-ray diffraction. The inorganic solid content was evaluated by Thermogravimetric Analysis (TGA). Photodegradation of Acid Orange 7 in aqueous solution was used a probe to assess the photocatalytic activity of the elaborated suspension under UV irradiation. The photokilling of Escherichia coli in presence of hybrid TiO₂ nanoparticles suspended in aqueous liquid under UV irradiation is evaluated. Such TiO₂ nanoparticles suspension shows a strong bactericidal activity with the total destruction of bacteria after only one hour.

Keywords

Anatase TiO2; Photocatalysis; Sol-Gel; Bacteria; Photokilling; Escherichia coli

Conflicts of Interest

The authors declare no conflicts of interest.

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