PCR-DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7


In order to test if the intestinal bacteria play an important role in antibacterial ability of earthworm, we chose Escherichia coli O157:H7, an anthropozoonosis pathogen, as a biological stressor and studied the change of intestinal bacteria community of earthworm by PCR-DGGE analysis. Results showed that the pathogen merely existed 1 - 3 days, then almost disappeared after through the earthworm’s gut. In this period, the diversity and abundance index of intestinal bacteria increased first, then decreased, and finally kept stably after 7 days. The result demonstrated that the intestinal bacteria of earthworm had ability of adjust community structure to eliminate the pathogen E. coli O157:H7, and the amount of bacteria Bacillus increased significantly, which might be the positive antagonism to E. coli O157:H7.

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Zhang, Y. , Wang, G. , Wu, Y. , Zhao, H. , Zhang, Y. and Sun, Z. (2013) PCR-DGGE analysis of earthworm gut bacteria diversity in stress of Escherichia coli O157:H7. Advances in Bioscience and Biotechnology, 4, 437-441. doi: 10.4236/abb.2013.43A058.

Conflicts of Interest

The authors declare no conflicts of interest.


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