Use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant Escherichia coli using PCR and future prospects with other bacterial genera: A pilot study

Subramanian Krishnan, Dheepikaa Balasubramanian, B. Appala Raju, Baddireddi Subadhra Lakshmi

Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, India.
Division of Infectious Diseases, Department of Pediatrics, The Saban Research Institute, Children’s Hospital Los Angeles, University of Southern California School of Medicine, Los Angeles, USA.
Tissue Culture and Drug Discovery Laboratory, Centre for Biotechnology, Anna University, Chennai, India.

DOI: 10.4236/abc.2012.24045                

Abstract

We developed a novel PCR method aimed at identi- fying and amplifying native codon sequences of muta- tion-prone amino acids in DNA gyrase implicated in quinolone resistance using a naturally occurring co- don bias in E. coli DNA gyrase A.

Keywords

Escherichia coli Gyrase A; Codon Bias; Mutation; Ciprofloxacin Resistance; PCR

Conflicts of Interest

The authors declare no conflicts of interest.

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