Use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant Escherichia coli using PCR and future prospects with other bacterial genera: A pilot study


We developed a novel PCR method aimed at identi- fying and amplifying native codon sequences of muta- tion-prone amino acids in DNA gyrase implicated in quinolone resistance using a naturally occurring co- don bias in E. coli DNA gyrase A.

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Krishnan, S. , Balasubramanian, D. , Raju, B. and Lakshmi, B. (2012) Use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant Escherichia coli using PCR and future prospects with other bacterial genera: A pilot study. Advances in Biological Chemistry, 2, 366-371. doi: 10.4236/abc.2012.24045.

Conflicts of Interest

The authors declare no conflicts of interest.


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